West China Journal of Stomatology

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Nasal Immunization with Co- expr ession Plasmid Harbor ing Genes Encoding Porphyromonas gingivalis FimA and Human Inter leukin- 15 in Mice

JIANG Guang- shui1, GUO Hong- mei1, WANG Xi- jun2, YANG Pi- shan1   

  1. 1. Dept. of General Dentistry, School of Stomatology, Shandong University, Jinan 250012, China; 2. Dept. of Temporomandibular Joint, West China College of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2007-04-25 Revised:2007-04-25 Online:2007-04-20 Published:2007-04-20
  • Contact: YANG Pi- shan,Tel:0531- 88382368

Abstract:

Objective To observe the antibody responses induced by recombinant plasmid pIRES- fimA:IL15 via nasal immunization to BABL/c mice and the regulation of IL- 15 to sIgA. Methods BABL/c mice were immunized with recombinant plasmids pIRES- fimA:IL15 and pIRES- fimA via nasal or intramuscular route. Serum IgG and salivary sIgA levels after immunization were analyzed by ELISA. Results Nasal immunization with plasmids pIRESfimA: IL15 or pIRES- fimA elicited significant higher level of salivary FimA- specific sIgA responses compared with intramuscular immunization. There was no significant difference of the serum IgG responses between nasal immunization mice and intramuscular immunization mice. Nasal immunization with plasmid pIRES - fimA:IL15 elicited significant higher level of salivary sIgA response than with pIRES- fimA(P<0.05). Conclusion Nasal dropping may be an effective mucosal immunization route of anti- Porphyromonas gingivalis DNA vaccine to elicit specific antibody responses in serum and oral region. IL- 15 has a positive regulation effect to sIgA response.

Key words: Porphyromonas gingivalis, periodontitis, mucosal immunity