Abstract:

Objective To observe the induction of apoptosis of cisplatin（DDP）to oral squamous cell carcinoma cell line （Tca8113） in vitro and study the role of Survivin on the apoptosis of Tca8113 cells induced by cisplatin. Methods The inhibitory effects of different doses of DDP on Tca8113 cells were assayed with MTT test. Apoptosis was determined by flow cytometry. The expression of Survivin was detected by RT- PCR and immunocytochemistry. Results Cisplatin obviously inhibited Tca8113 cells growth in a dose and time dependent manner. The apoptotic index showed the similar trend. Survivin gene expression was decreased with increasing of time and reached the lowest level at 24 hours after DDP treatment, then increased after that time. Conclusion Cisplatin gene can effectively induce apoptosis in Tca8113 cells and the inhibition of Survivin gene expression may play a critical role on Tca8113 cell apoptosis induced by cisplatin.

Key words: Survivin, cisplatin, tongue squamous cell carcinoma, apoptosis