Abstract:

 Objective Using MBECTM-Assay to assay minimal hiof'ilm eradication concentratio( MBEC) of Galla Chinensis and Nidus Vespae to oral bacterial hiof'ilm. To set up traditional Chinese medicine susceptibility pharmacodynamic empirical study methods of oral bacterial biofilm. Methods  Cariogenic bacteria strains were selec:tecl Streptococcus mutan_s ATCC 25175, Streptococcu_s .sangui.s ATCC 10556. Lactobacillus rhamnosus AC 413. Actinomyces naeslundii WVU 627) in this study. Extraction components of Galla Chinensis were CCE( aqueous extract),  GJCE -B( 30%  alcohol  extract)  and  extraction  components  of Nidus  Vespae  were  NVEI( 95%  alcohol extract). LI)To observe oral bacterial biof'ilm formatiom in MBECTM一Device at diff'erent time.(②MBECTM-HTP-Assay: The minimal inhibitory concentration( MIC) and minimal biofilm eradication concentration( MBEC) of' (GCE. GJCE-B and NVEI to oral bacteria strains were determined. Results Oral bacterial biofilm were readily formed on the lid of MBECm'_Device under selected condition observed by SEM). Oral cariogenic hacteria growing as plankton populations were sensitive to GCE. GCE-B and NVEI. To GCE. GCE-B and NVEl. oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing plankton bacteria. GCE and GCE-B were the most eff'ective medicine against oral cariogenic hacterial biof'ilm. NVEl were effective in killing oral-bacterial biofilm at relatively high concentration. Conclusion  GCE and GCE-B were effective medicine against oral cariogenic bacterial biofilm. MBEC( minimal biof'ilm eradication concentration) can provide a relative accurate medicine concentration f'or clinical test.

Key words: GallaChinesis, NidusVespae, oralbacterialbiofilm, caries