West China Journal of Stomatology

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Compar e the Effect of Lipo- and Normal Pr epar ed Sodium Mor rhuate on ECV- 304 Cell Line

TU Junbo1, LAN Hai - long1, YANG Zhuang- qun1, ZHANG Tie - liang2, SONG Yong2, XING Zhe1   

  1. 1. Dept. of Oral and Maxillary Surgery, College of Stomatology, Xi′an Jiaotong University, Xi′an 710004, China;2. Research Center for Stomatology, College of Stomatology, Xi′an Jiaotong University, Xi′an 710004, China
  • Received:2006-12-25 Revised:2006-12-25 Online:2006-12-20 Published:2006-12-20
  • Contact: YANG Zhuang- qun,Tel:029- 87256632
  • About author:

Abstract:

Objective To compare the effect of Sodium Morrhuate on ECV- 304 between its lipo- and normal preparation. Methods The ECV- 304 cell line was supplemented with Sodium Morrhuate and lipo- Sodium Morrhuate in order, and the result on morpholog(y microscope, Giemsa Staining and electron microscope), cell activi(ty MTT), and flow cytometer between the two preparation were compared. Results In normal preparation group, cell′s edema occurred. Chromatin was like catkins. Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared. In lipo- Sodium Morrhuate group, the membrane was creased and processus appeared. Chromatin aggregates to the membrane of nucleus was like crescent, and then broken. The apoptotic body was formed. MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively, which cues the vital cells decreased sharply. The curve of lipo- Sodium Morrhuate group was gentle and the change time was long relatively, which cues the vital cells decreased slowly. The flow cytometer showed that typical apoptosis peak appeared. Conclusion The normal preparation group shows an acute toxic effect on ECV- 304 cell line, which result in a necrosis course, while lipo- Sodium Morrhuate shows a gradual releasing process, which may indicate a apoptosis course.

Key words: Sodium Morrhuate, liposomes, hemangioma, ECV- 304 cell line