Abstract:

Objective  To investigate the possibility of adipose-derived stromal cells（ADSCs） transfeced by ade-novirus containing human bone morphogenetic protein-2（Ad-hBMP-2） gene and their osteogenic potential. Methods ADSCs were obtained from inguinal fat tissue of 4 weeks old SD rats. After exposure to adenovirus containing green fluorescent protein（Ad-GFP）, fluorescent microscope was used to observe gene transfection effect once 12 hours. After transfected with Ad-hBMP-2，cytochemistry, immucytochemistry and Western blot were used to examine the ex- pression of  alkaline phosphatase（ALP）, osteocalcin（OC） and hBMP-2. Results  After exposed to Ad-GFP 12 hours, 52% ADSCs were observed being transfected and 48 hours later reached 95%. The double number time belonged after transfecting with Ad-hBMP-2, and cytochemistry, immucytochemistry and Western blot examines indicated positive results of ALP, OC, hBMP-2 after 48 hours. Conclusion  Adipose tissue contains abundant ADSCs which could be transfected as gene vectors by adenovirus, ADSCs transfected with Ad-hBMP-2 can convert to ostoeblasts, and can act as a kind of seed cells for osteo-tissue engineering. 

Key words: adipose tissue-derived stromal cells, bone morphogenetic protein-2, adenovirus, gene transfection