West China Journal of Stomatology

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Immortalized Human Odontoblast-like Cell Line Expressed Dentin Extracellular Matrixin vitro

WANGHan-guo,XIAO Ming-zhen,ZHAO Shou-liang,HAO Jian-jun,ZHANG Min   

  1. Dept.ofOperative Dentistry and Endodontics,College ofStoma- tology,The Fourth MilitaryMedical University,Xi′an710032,China
  • Received:2005-12-25 Revised:2005-12-25 Online:2005-12-20 Published:2005-12-20

Abstract: Objective To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-lin vitro.Methods Collagen typeⅠ, bone sialoprotein (BSP),dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein(DSP) were detected in these cells by immunohistoche- mistry, RT-PCR and in situ hybridization. During being cultured in mineralizingmedium for 5 weeks, the secretion of OC and ac- tivity of ALP were measured once aweek.Results DSPP, DMP1,BSP and collagen typeⅠwere expressed in hTERT-hOd-l ei- ther at mRNA or protein level. Underthe induction of mineralizingmedium, the cells showed higher activity of ALP and increased secretion of OC.Conclusion hTERT-hOd-l expressed dentin extracellularmatrixin vitro, which means the cell line has the po- tential of mineralization.

Key words: odontoblast, dentin extracellular matrix, mineralization