Abstract:

Objective　To observe the differentmRNAlevels ofCandida albicansALS gene family between planktonic and bio- film-grown cells.Methods　ATCC 90038 and a wild strain of Candida albicans, biofilm modelsin vitrowere formed on glass slides. After 48 hours′incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.Results　The amounts ofALS1 and ALS4 mRNAof thewild strain in biofilm in- creased comparing with planktonic cells, while ATCC 90038 didn′t.Conclusion　The members ofALS gene familymay play im- portant roles in the course ofCandida albicansbiofilm formation.

Key words: Candida albicans, biofilm, ALS gene