Effect of Hypoxia on the Expression of Urokinase-type Plasminogen Activator in Human Tongue Squamous Cell Carcino- ma Cells (Tca8113)

West China Journal of Stomatology

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Effect of Hypoxia on the Expression of Urokinase-type Plasminogen Activator in Human Tongue Squamous Cell Carcino- ma Cells (Tca8113)

WANG Sheng-zhi1,LIANGXin-hua2,CHENG Zhou1,BIHong-guang1,MAO Zu-yi2,WANG Da-zha- ng2

1.Dept.ofOral andMaxillofacial Surgery,The Affiliated Yantai YuhuangdingHospital ofQingdao UnivercityMedical Col- lege,Yantai264000,China;2.Key.Laboratory of Oral Biomedical Engineering Ministry of Education,Sichuan University, Chengdu610041,China

Received:2005-06-25 Revised:2005-06-25 Online:2005-06-20 Published:2005-06-20

Abstract

Abstract:

Objective　To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.Methods　Under different hypoxic times(0,3,6,12,24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).Results　Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.Conclusion　Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.

Key words: hypoxia, urokinase-type plasminogen activator, tongue squamous cell carcinoma, invasion, metastasis

WANG Sheng-zhi1,LIANGXin-hua2,CHENG Zhou1,BIHong-guang1,MAO Zu-yi2,WANG Da-zha- ng2. Effect of Hypoxia on the Expression of Urokinase-type Plasminogen Activator in Human Tongue Squamous Cell Carcino- ma Cells (Tca8113)[J]. West China Journal of Stomatology.

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Effect of Hypoxia on the Expression of Urokinase-type Plasminogen Activator in Human Tongue Squamous Cell Carcino- ma Cells (Tca8113)

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