Abstract:

Objective To investigate the variety of proliferating ability of umbilical endothelia《UE) transfected场plasmid pBABE-HYGR-hTERT. Methods UE~identified from two aspects: morphology and CD34 labeling technique. The plasmid ~obtained and identified场alkali splitting and gel electrophoresis. Uposomes~used to transfect UE. RT-PCR based telo- meric repeat amplification protocol(TRAP) assay~used to measure the telomerase activity of endothelia. Results LlE arranged as "cobblestone" and were positive of CD34 labeling. Endothelia transfected场pBABE-HYGR-hTERT(HC ) had an raised absor- bance of 0.889. The shape of growth。~of HC was similar to UE. But the absorbance of MTT test and the amount of HC were prior to UE at every measuring time and the amount of HC increased four times within 8 days ( P<0.05).Conclusion The transfection of pBABE-HYGRO-hTERT had greatly improved the prliferating abilities and activated the telemerase of UE.

Key words: vascular endothelium, catalytic subunit of telemerase