West China Journal of Stomatology
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LIPeng*,XUYan,ZHANG Yunhui,et al.
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Abstract:
Objective The purpose of this study was to construct a eukaryote expression vector carrying human sIL-1R gene.Methods Both sIL-1R gene and plasmid pcDNA3·1(+) DNAwere digestedwithKpnIandXhoI. After purification, the two fragments obtained were ligated by usingTakaRa DNALigation Kit. This recombinant DNAwas then transformed intoE.coli Competent Cells JM109 and positive cloneswere selected on the LB agarose plate containingAmpicillin (80μg/ml).Results Six single clones were identified by double digestionwithKpnIandXhoI, and two fragmentswith the size of 5·4 kb and 1·0 kbwere produced as expected.Conclusion The sIL-1R gene was successfully inserted into the eukaryote expression vector plasmid pcDNA 3·1(+) by the recombination techniquein vitro.
Key words: human soluble interleukin-1 receptor, eukaryote expression vector, plasmid pcDNA 3·1(+)
LIPeng*,XUYan,ZHANG Yunhui,et al.. Construction of Eukaryote Expression Vector Carrying Human Soluble Interleukin-1 Receptor Gene[J]. West China Journal of Stomatology.
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