Abstract:

Objective　The purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG).Methods　PCR was performed to amplify the AMG encoding region. Amplified fragments for human AMG were recov- ered and inserted into eukaryotic expression vectors PsecTaq2A. The recombinant plasmid PsecTaq2A-AMG was constructed and their positive cloneswere identified.Results　①Amplified productswere checked by electrophoresis and the resultswere satisfac- tory.②The recombinant plasmid PsecTaq2A-AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistentwith those fromGenBank.Conclusion　The recombinant plasmid PsecTaq2A-AMGwas suc- cessfully constructed with properly inserted DNA sequence encoding mature amelogenin.

Key words: human amelogenin, recombinant plasmid, PsecTaq2A-AMG