Effect of tumor-stromal fibroblasts on the biological behavior of salivary gland pleomorphic adenoma cells in vitro

doi:10.7518/hxkq.2023.2022314

Abstract

Abstract:

Objective This study aims to investigate the effects of tumor-stromal fibroblasts (TSFs) on the proliferation, invasion, and migration of salivary gland pleomorphic adenoma (SPA) cells in vitro. Methods Salivary gland pleomorphic adenoma cells (SPACs), TSFs, and peri-tumorous normal fibroblasts (NFs) were obtained by tissue primary culture and identified by immunocytochemical staining. The conditioned medium was obtained from TSF and NF in logarithmic phase. SPACs were cultured by conditioned medium and treated by TSF (group TSF-SPAC) and NF (group NF-SPAC). SPACs were used as the control group. The proliferation, invasion, and migration of the three groups of cells were detected by MTT, transwell, and scratch assays, respectively. The expression of vascular endothelial growth factor (VEGF) in the three groups was tested by enzyme linked immunosorbent assay (ELISA). Results Immunocytochemical staining showed positive vimentin expression in NF and TSF. Results also indicated the weak positive expression of α-smooth muscle actin (SMA) and fibroblast activation protein (FAP) in TSFs and the negative expression of α-SMA and FAP in NFs. MTT assay showed that cell proliferation in the TSF-SPAC group was significantly different from that in the NF-SPAC and SPAC groups (P<0.05). Cell proliferation was not different between the NF-SPAC and SPAC groups (P>0.05). Transwell and scratch assays showed no difference in cell invasion and migration among the groups (P>0.05). ELISA showed that no significant difference in VEGF expression among the three groups (P>0.05). Conclusion TSFs may be involved in SPA biological behavior by promoting the proliferation of SPACs but has no effect on the invasion and migration of SPACs in vitro. Hence, TSF may be a new therapeutic target in SPA treatment.

Key words: salivary pleomorphic adenoma, tumor-stromal fibroblast, proliferation, invasion, migration, borderline tumor

CLC Number:

Hou Yali, Li Hexiang, Song Peng, Yang Yanxiao, Hao Yali, Liu Huijuan. Effect of tumor-stromal fibroblasts on the biological behavior of salivary gland pleomorphic adenoma cells in vitro[J]. West China Journal of Stomatology, 2023, 41(2): 149-156.

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组别	细胞侵袭数	P值
NF-SPAC	21.30±5.10	0.612
TSF-SPAC	20.89±5.35
SPAC	23.20±5.51

组别	T0	T1	T0-T1	P值
NF-SPAC	1 770.17±17.42	1 570.17±27.58	200.00±34.00	0.098
TSF-SPAC	1 759.33±33.95	1 483.50±33.27	275.83±42.11
SPAC	2 107.67±27.27	1 774.00±33.03	333.67±49.65

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