Aging effect of osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in human periodontal ligament cells under continuous static pressure

doi:10.7518/hxkq.2022.06.005

Abstract

Abstract:

Objective The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in human periodontal ligament cells (HPDLCs) was investigated by cell culture under continuous static pressure. Methods HPDLCs were primarily cultured by tissue explant method and divided into three groups: group A (13-18 years old), group B (19-29 years old), and group C (30-44 years old). CCK-8 was used to detect the proliferation of HPDLCs. The senescence of HPDLCs was detected by senescence-associated β-galactosidase staining. Cells in the three groups were respectively given 0, 1.5, 3, 6, 12, 24, 48, and 72 h of continuous static pressure in vitro. The expression of OPG and RANKL in the supernatant was detected by enzyme-linked immunosorbent assay. Results After continuous static pressure in vitro for stimulation, the expression of OPG and RANKL changed. The expression of OPG increased with time and age (P<0.01). The expression of RANKL increased with time and decreased with age (P<0.01). The ratio of OPG/RANKL initially decreased, increased with time, and then continued to rise with age (P<0.01). Conclusion Aging could increase the expression of OPG and the ratio of OPG/RANKL and decrease the expression of RANKL in HPDLCs under continuous static pressure in vitro.

Key words: aging, orthodontics, human periodontal ligament cells, continuous static pressure, osteoprotege-rin, receptor activator of nuclear factor-κB ligand

CLC Number:

R 783.5

Wu Jie, Cui Zhanqin, Han Yu, Li Wenjing. Aging effect of osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in human periodontal ligament cells under continuous static pressure[J]. West China Journal of Stomatology, 2022, 40(6): 654-661.

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References 16

1	Khosla S. Minireview: the OPG/RANKL/RANK system[J]. Endocrinology, 2001, 142(12): 5050-5055.
2	Yamaguchi M. RANK/RANKL/OPG during orthodontic tooth movement[J]. Orthod Craniofac Res, 2009, 12(2): 113-119.
3	Henneman S, Von den Hoff JW, Maltha JC. Mechanobio-logy of tooth movement[J]. Eur J Orthod, 2008, 30(3): 299-306.
4	Hasegawa T, Yoshimura Y, Kikuiri T, et al. Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells[J]. J Periodontal Res, 2002, 37(6): 405-411.
5	Nettelhoff L, Grimm S, Jacobs C, et al. Influence of mechanical compression on human periodontal ligament fibroblasts and osteoblasts[J]. Clin Oral Investig, 2016, 20(3): 621-629.
6	Simonet WS, Lacey DL, Dunstan CR, et al. Osteoprotegerin: a novel secreted protein involved in the regulation of bone density[J]. Cell, 1997, 89(2): 309-319.
7	Harman D. Aging: overview[J]. Ann N Y Acad Sci, 2001, 928(1): 1-21.
8	Kanzaki H, Chiba M, Shimizu Y, et al. Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor κB ligand up-regulation via prostaglandin E2 synthesis[J]. J Bone Mineral Res, 2002, 17(2): 210-220.
9	黄生高, 张建兴, 熊培颖, 等. 持续静压力对人牙周膜细胞OPG及ODF mRNA表达的影响[J]. 临床口腔医学杂志, 2006, 22(6): 347-350.
	Huang SG, Zhang JX, Xiong PY, et al. Effect of continuously compressive pressure on the expression of OPG and ODF mRNA in human periodontal ligament cells in vitro [J]. J Clin Stomatol, 2006, 22(6): 347-350.
10	Kawasaki K, Takahashi T, Yamaguchi M, et al. Effects of aging on RANKL and OPG levels in gingival crevicular fluid during orthodontic tooth movement[J]. Orthod Craniofac Res, 2006, 9(3): 137-142.
11	刘艳, 刘月华, 陈玉荣, 等. 增龄因素对大鼠正畸牙齿移动中牙周组织内RANKL因子表达的影响[J]. 江苏医药, 2011, 37(23): 2758-2760.
	Liu Y, Liu YH, Chen YR, et al. Effect of aging on RANKL expression in periodontal tissue of rat during orthodontic tooth movement[J]. Jiangsu Med, 2011, 37(23): 2758-2760.
12	George P, George J K, Krishnan V, et al. Periodontal li-gament cells in adolescents and adults: Genetic level responses to orthodontic forces[J]. Am J Orthod Dentofacial Orthop, 2020, 158(6): 816-823.
13	张瑞林, 孙瑶. 正畸牙槽骨改建中RANKL/OPG表达动态变化特征及牙周组织细胞凋亡观察[J]. 口腔医学, 2019, 39(6): 488-493.
	Zhang RL, Sun Y. Dynamic changes of RANKL/OPG expression and periodontal cell apoptosis in orthodontic alveolar bone reconstruction[J]. J Stomatol, 2019, 39(6): 488-493.
14	柏思羽, 陈悦, 戴红卫, 等. 机械压应力下骨硬化蛋白对成牙骨质细胞功能影响及机制的体外研究[J]. 华西口腔医学杂志, 2019, 37(2): 162-167.
	Bai SY, Chen Y, Dai HW, et al. Effect of sclerostin on the functions and related mechanisms of cementoblasts under mechanical stress[J]. West China J Stomatol, 2019, 37(2): 162-167.
15	Lee YH, Nahm DS, Jung YK, et al. Differential gene expression of periodontal ligament cells after loading of static compressive force[J]. J Periodontol, 2007, 78(3): 446-452.
16	Cao H, Kou X, Yang R, et al. Force-induced Adrb2 in periodontal ligament cells promotes tooth movement[J]. J Dent Res, 2014, 93(11): 1163-1169.

加力时间/h		空白对照组		压力组
加力时间/h	A组	B组	C组	A组	B组	C组
0	5.572 0±0.347 4	5.845 4±0.445 1	5.521 6±0.196 9	5.571 4±0.179 6	5.393 2±0.339 6	5.414 9±0.584 3
1.5	7.866 4±0.976 6	8.499 1±0.962 6	9.899 7±0.843 3	15.221 4±0.714 3	15.608 5±0.947 9	16.026 6±0.229 2
3	12.771 9±0.754	14.372 8±0.516 0	15.606 0±0.605 0^a	29.084 1±0.930 4	30.479 7±0.455 6	31.461 7±0.496 7
6	20.153 3±1.967 5	23.958 3±1.044 7^a	25.938 6±1.3967 ^a	33.594 7±1.175 3	38.826 7±1.991 5^a	40.096 1±0.740 6^a
12	37.565 4±1.529 6	40.235 7±1.711 2^a	42.015 8±1.996 7^a	89.030 9±1.384 9	92.129 1±1.517 5^a	97.437 3±1.609 0^ab
24	59.576 6±1.804 9	65.217 5±1.443 9^a	71.085 0±1.644 1^ab	118.093 8±1.172 1	124.568 2±3.128 9^a	133.845 6±1.492 3^ab
48	73.786 7±2.926 9	77.833 1±1.850 0^a	80.485 9±1.644 3^ab	162.248 3±3.692 5	169.217 1±2.799 3^a	174.224 2±1.938 0^ab
72	84.806 0±1.875 5	90.283 1±1.679 8^a	96.120 2±1.436 2^ab	224.321 6±1.799 0	229.489 3±1.606 0^a	235.264 5±2.483 3^ab

加力时间/h	空白对照组			压力组
加力时间/h	A组	B组	C组	A组	B组	C组
0	0.272 8±0.016 9^cdefg	0.266 4±0.016 9^cdefg	0.284 1±0.016 9^defd	0.278 3±0.015 2	0.239 6±0.011 6	0.233 6±0.016 9
1.5	0.340 4±0.026 6^cdefg	0.314 7±0.044 2^cdefg	0.306 8±0.066 3^defg	0.948 5±0.011 7	0.870 8±0.073 0	0.647 8±0.187 9
3	0.533 8±0.059 7^defg	0.493 1±0.024 0^defg	0.458 8±0.053 1^defg	2.580 0±0.099 4	1.867 6±0.175 5	1.503 2±0.159 6^a
6	0.908 6±0.159 6	0.848 2±0.226 2	0.775 5±0.257 1	4.749 3±0.138 3	3.396 0±0.787 2^a	2.857 8±0.515 2^a
12	1.6608±0.267 0^cef	1.350 8±0.254 4^ef	1.195 9±0.242 9	6.893 3±0.754 3	5.515 7±0.891 5^a	5.052 6±0.701 2^a
24	2.620 5±0.597 2^cdf	2.482 7±0.664 0^cdf	2.140 1±0.337 1^cd	8.889 2±0.714 8	6.458 1±0.402 6^a	5.390 3±0.992 6^ab
48	3.109 3±0.434 9^cd	2.759 4±0.334 9^cd	2.595 9±0.223 5^acd	10.003 4±0.864 6	7.546 9±0.854 6^a	6.185 1±0.376 6^ab
72	3.425 3±0.342 4^cde	3.289 7±0.611 0^cdef	2.952 0±0.453 0^cde	11.602 8±0.768 5	8.386 9±0.291 8^a	7.182 3±0.722 1^ab

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