Salivary biochemical indices related to early childhood caries

doi:10.7518/hxkq.2021.03.009

Abstract

Abstract: Objective

This study aimed to compare the salivary biochemical indices between caries-free individuals and those with early childhood caries (ECC), and construct a saliva-based caries diagnostic model by analyzing the correlation between salivary biochemical indices and caries severity.

Methods

A total of 120 children aged 4-6 years were selected and divided into two groups: individuals with ECC (C group, n=60) and healthy children (H group, n=60). Salivary samples were collected to compare the pH, total protein, and ion concentrations between the two groups. The correlation between the salivary biochemical indices and caries severity was examined, and an ECC diagnostic model was established.

Results

The NO₃^- concentration significantly decreased in the C group, whereas the Cl^-, Br^-, NH₄⁺, and Mg²⁺ concentrations significantly increased in the C group (P<0.05). In addition, the salivary caries severity had a significantly negative correlation with the NO₃^- concentration but had a positive correlation with Br^-, Cl^-, and NH₄⁺ concentrations (P<0.05). The ECC diagnostic model based on salivary biochemical indices could yield satisfactory results in terms of distinguishing the C and H groups with over 85% accuracy.

Conclusion

Salivary biochemical indices can contribute to the diagnosis and risk assessment of ECC.

Key words: early childhood caries, saliva, biochemical indices, correlation, diagnosis model

CLC Number:

R 781.1

Zhang Ying, Jia Songbo, Li Fan, Li Shanshan, Zhang Lijuan, Tan Kaixuan, Lu Jie, Yang Fang. Salivary biochemical indices related to early childhood caries[J]. West China Journal of Stomatology, 2021, 39(3): 300-305.

Figures/Tables 6

Tab 1

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References 30

1	Drury TF, Horowitz AM, Ismail AI, et al. Diagnosing and reporting early childhood caries for research purpo-ses. A report of a workshop sponsored by the National Institute of Dental and Craniofacial Research, the Health Resources and Services Administration, and the Health Care Financing Administration[J]. J Public Health Dent, 1999, 59(3): 192-197.
2	Du MQ, Li Z, Jiang H, et al. Dental caries status and its associated factors among 3- to 5-year-old children in China: a national survey[J]. Chin J Dent Res, 2018, 21(3): 167-179.
3	Pierce A, Singh S, Lee J, et al. The burden of early childhood caries in Canadian children and associated risk factors[J]. Front Public Health, 2019, 7: 328.
4	Nadelman P, Bedran N, Magno MB, et al. Premature loss of primary anterior teeth and its consequences to primary dental arch and speech pattern: a systematic review and meta-analysis[J]. Int J Paediatr Dent, 2020, 30(6): 687-712.
5	Dawes C. Salivary flow patterns and the health of hard and soft oral tissues[J]. J Am Dent Assoc, 2008, 139(): 18S-24S.
6	Hemadi AS, Huang R, Zhou Y, et al. Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment[J]. Int J Oral Sci, 2017, 9(11): e1.
7	Gomez J. Detection and diagnosis of the early caries lesion[J]. BMC Oral Health, 2015, 15 (): S3.
8	Abrams SH, Sivagurunathan KS, Silvertown JD, et al. Correlation with caries lesion depth of the canary system, DIAGNOdent and ICDAS Ⅱ[J]. Open Dent J, 2017, 11: 679-689.
9	Pyati SA, Naveen Kumar R, Kumar V, et al. Salivary flow rate, pH, buffering capacity, total protein, oxidative stress and antioxidant capacity in children with and without dental caries[J]. J Clin Pediatr Dent, 2018, 42(6): 445-449.
10	Sekhri P, Sandhu M, Sachdev V, et al. Estimation of trace elements in mixed saliva of caries free and caries active children[J]. J Clin Pediatr Dent, 2018, 42(2): 135-139.
11	World Health Organization. Oral health surveys:basic methods[M]. 5th ed. Geneva: World Health Organization, 2013: 35-55.
12	胡炜, 杜东娜, 王敬楠. 离子色谱仪的操作过程[J]. 国际感染病学(电子版), 2019, 8(1): 177-178.
	Hu W, Du DN, Wang JN. Operation process of ion chromatograph[J]. Inf Int (Electr Ed), 2019, 8(1): 177-178.
13	Humphrey SP, Williamson RT. A review of saliva: normal composition, flow, and function[J]. J Prosthet Dent, 2001, 85(2): 162-169.
14	Denny P, Hagen FK, Hardt M, et al. The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions[J]. J Proteome Res, 2008, 7(5): 1994-2006.
15	Kim J, Seo BS. How to calculate sample size and why[J]. Clin Orthop Surg, 2013, 5(3): 235-242.
16	Hegde AM, Neekhra V, Shetty S. Evaluation of levels of nitric oxide in saliva of children with rampant caries and early childhood caries: a comparative study[J]. J Clin Pediatr Dent, 2008, 32(4): 283-286.
17	Syed M, Sachdev V, Chopra R. Intercomparison of salivary nitric oxide as a biomarker of dental caries risk between caries-active and caries-free children[J]. Eur Arch Paediatr Dent, 2016, 17(4): 239-243.
18	Doel JJ, Hector MP, Amirtham CV, et al. Protective effect of salivary nitrate and microbial nitrate reductase activity against caries[J]. Eur J Oral Sci, 2004, 112(5): 424-428.
19	Duncan C, Dougall H, Johnston P, et al. Chemical gene-ration of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate[J]. Nat Med, 1995, 1(6): 546-551.
20	Sánchez GA, Miozza VA, Delgado A, et al. Total salivary nitrates and nitrites in oral health and periodontal disease[J]. Nitric Oxide, 2014, 36: 31-35.
21	Hegde MN, Attavar SH, Shetty N, et al. Saliva as a biomarker for dental caries: a systematic review[J]. J Conserv Dent, 2019, 22(1): 2-6.
22	Silletti E, Vingerhoeds MH, Norde W, et al. Complex formation in mixtures of lysozyme-stabilized emulsions and human saliva[J]. J Colloid Interface Sci, 2007, 313(2): 485-493.
23	LeGeros RZ. Calcium phosphates in oral biology and medicine[J]. Monogr Oral Sci, 1991, 15: 1-201.
24	Bhalla S, Tandon S, Satyamoorthy K. Salivary proteins and early childhood caries: a gel electrophoretic analysis[J]. Contemp Clin Dent, 2010, 1(1): 17-22.
25	Gao X, Jiang S, Koh D, et al. Salivary biomarkers for dental caries[J]. Periodontol 2000, 2016, 70(1): 128-141.
26	Singh S, Sharma A, Sood PB, et al. Saliva as a prediction tool for dental caries: an in vivo study[J]. J Oral Biol Craniofac Res, 2015, 5(2): 59-64.
27	闫爱云, 雷守成, 张鲜桃. 离子色谱技术的进展[J]. 疾病监测与控制, 2015, 9(10): 713-715.
	Yan AY, Lei SC, Zhang XT. Progress of use on ion chrom atography[J]. J Dis Monit Contr, 2015, 9(10): 713-715.
28	Bohn AA, de Morais HA. A quick reference on chloride[J]. Vet Clin North Am Small Anim Pract, 2017, 47(2): 219-222.
29	Shekarabi M, Zhang J, Khanna AR, et al. WNK kinase signaling in ion homeostasis and human disease[J]. Cell Metab, 2017, 25(2): 285-299.
30	Gröber U, Schmidt J, Kisters K. Magnesium in prevention and therapy[J]. Nutrients, 2015, 7(9): 8199-8226.

唾液参数	C组	H组	P值
NO₃^-/（mg·L^-1）	10.90±13.18	32.93±66.46	***
Cl^-/（mg·L^-1）	599.52±173.05	471.25±100.80	***
NH₄⁺/（mg·L^-1）	197.34±67.21	159.34±56.82	*
Mg²⁺/（mg·L^-1）	3.51±1.98	3.01±2.24	*
Br^-/（mg·L^-1）	2.26±1.47	1.29±0.75	***
NO₂^-/（mg·L^-1）	2.44±3.12	3.48±4.75	NS
总蛋白/（mg·L^-1）	1 173.89±325.35	1 116.75±332.62	NS
K⁺/（mg·L^-1）	785.73±246.77	731.54±188.63	NS
PO₄^3-/（mg·L^-1）	468.48±166.66	421.10±126.18	NS
Na⁺/（mg·L^-1）	100.61±64.77	76.86±29.57	NS
F^-/（mg·L^-1）	32.19±18.05	28.59±14.73	NS
SO₄^2-/（mg·L^-1）	21.96±9.49	18.17±5.92	NS
Ca²⁺/（mg·L^-1）	14.40±6.55	10.92±6.37	NS
pH	6.85±0.20	6.85±0.23	NS

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