West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (1): 26-31.doi: 10.7518/hxkq.2021.01.004

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MicroRNA-146a regulates the production of cytokines in lymphocytes stimulated by Porphyromonas gingivalis lipopolysaccharide

Si Yuting1,2(), Song Jinhua1,2, Fang Zhen2, Han Xiaozhe3, Jiang Shaoyun1,2()   

  1. 1.Dept. of Periodontology, Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China
    2.Dept. of Periodontology, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China
    3.The Forsyth Institute, Harvard School of Dental Medicine, Cambridge 02146, USA
  • Received:2020-02-17 Revised:2020-10-23 Online:2021-02-01 Published:2021-03-02
  • Contact: Jiang Shaoyun E-mail:siyutingsyt401@163.com;jiangshaoyun11@126.com
  • Supported by:
    The National Natural Science Foundation of China(81670990);Basic Research Grand of Peking University Shenzhen Hospital(JCYJ2019004RC);National Institute of Dental and Craniofacial Research, The United States of America(R01DE025255)

Abstract: Objective

This study aimed to investigate the effects of microRNA-146a (miR-146a) on the production of cytokines in lymphocytes stimulated by Porphyromonas gingivalis (P.gingivalis) lipopolysaccharide (LPS).

Methods

Lymphocytes were harvested from mouse spleen and cultured in vitro. The cells were treated with P. gingivalis LPS, miR-146a mimic, or miR-146a inhibitor. Scramble RNA served as the negative control of mimic and inhibitor. The production of inflammatory cytokines was detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.

Results

Compared with non-LPS-stimulated group, P. gingivalis LPS could increase the levels of interleukin (IL)-1β, IL-6, receptor activator NF-κB ligand (RANKL), and IL-10 (P<0.05) and decrease the mRNA level of osteoprotectin (OPG) (P<0.05). However, it did not significantly change the secretion of OPG. Compared with the negative control group, miR-146a mimic upregulated the levels of IL-10 and OPG (P<0.05), downregulated IL-1β, IL-6, and RANKL (P<0.05). Meanwhile, miR-146a inhibitor had a reverse effect on these cytokines (P<0.05) in P.gingivalis LPS-treated-lymphocytes.

Conclusion

MiR-146a can provide a suitable microenvironment for bone formation by preventing the inflammatory effects of P.gingivalis LPS through the inhibition of IL-1β, IL-6, and RNAKL, thereby enhancing IL-10 and OPG.

Key words: periodontitis, microRNA-146a, cytokine, lymphocytes, lipopolysaccharide

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