West China Journal of Stomatology ›› 2020, Vol. 38 ›› Issue (2): 128-132.doi: 10.7518/hxkq.2020.02.003

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Temporal and spatial distribution of Gli1+ cells and their function during periodontal development

Wang Yun1, Xie Xudong2, Xu Chunmei2, Wang Jun2()   

  1. 1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2019-10-15 Revised:2020-01-08 Online:2020-04-01 Published:2020-04-15
  • Contact: Jun Wang E-mail:junwang@scu.edu.cn
  • Supported by:
    The National Natural Science Foundation of China(81700980);Sichuan Province Science and Technology Support Program(2019YJ0097);China Postdoctoral Science Foundation(2017M623048);Postdoctoral Research Foundation of Sichuan University(2018SCU12018)

Abstract:

Objective This study aimed to investigate the distribution of Gli1 + cells in the periodontal ligament (PDL) and to evaluate their contribution in the development of periodontal tissue by using transgenic mouse lines. Methods Gli1 lacZ/+ mice were harvested at different ages (3, 6, and 8 weeks), and the temporal and spatial distribution patterns of Gli1 +PDL cells were revealed by X-gal staining. Afterward, 3-week-old Gli1-Cre ERT2/+;R26R tdTomato/+mice were administered with tamoxifen, and the fates of Gli1 +cells and their descendants were traced during periodontal development. Results A large number of Gli1 +cells were detected in the PDL of the 3-week-old mice; however, their number significantly decreased from 3 weeks to 8 weeks (P<0.05). Cell lineage tracing data showed that the descendants of Gli1 + cells dramatically increased from 3 weeks to 8 weeks (P<0.05) and gradually differentiated into fibroblasts, cementocytes, and osteocytes. Conclusion The multi-differentiation potential of Gli1 +PDL cells was revealed, indicating that Gli1 +cells are an important cell source for periodontal development.

Key words: Gli1, periodontal tissue, development, lineage tracing, stem cell

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