Effects of Bruton’s tyrosine kinase on the proliferation and differentiation of osteoclasts

doi:10.7518/hxkq.2019.04.004

Abstract

Abstract:

Objective To observe the effect of Bruton’s tyrosine kinase (BTK) on the proliferation and differentiation of osteoclasts and to explore the mechanism of BTK on bone destruction in periapical periodontitis. Methods After RAW264.7 cells induced with 100 ng·L ^-1 receptor activator for nuclear factor-κB ligand (RANKL) for 5 days, osteoclast induction was confirmed by light microscopy, tartrate-resistant acid phosphatase (TRAP) staining, and quantitative real-time PCR (RT-qPCR). Then, BTK-small interfering RNA (BTK-siRNA) was transfected into cells induced for 5 days. After 24 h, the expression of TRAP mRNA was measured using RT-qPCR, and the proliferation and differentiation of osteoclasts were detected using CCK-8 and TRAP activity assay. Statistical analysis was performed. Results After RAW264.7 was induced with RANKL for 5 days, a large number of round, ellipse, irregularly protuberant, and TRAP-positive macrophages were observed under light microscopy. The expression of TRAP mRNA significantly reduced after 24 h of BTK-siRNA transfection (P<0.05). The detection of CCK-8 and TRAP activities showed that the proliferation and differentiation of osteoclasts significantly decreased (P<0.05). Conclusion Silencing of BTK can inhibit the proliferation and differentiation of osteoclasts. BTK can be used as a new target for the inhibition of osteoclasts.

Key words: osteoclast, Bruton’s tyrosine kinase;, bone destruction, small interfering RNA transfection

CLC Number:

R781

Hong Zhang,Lina Wang,Meina Zuo,Ming Dong,Dongmei Shi,Huijun Xu,Weidong Niu. Effects of Bruton’s tyrosine kinase on the proliferation and differentiation of osteoclasts[J]. West China Journal of Stomatology, 2019, 37(4): 361-365.

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