Rat model with oral submucous fibrosis induced by arecoline and mechanical stimulation

doi:10.7518/hxkq.2019.03.006

Abstract

Abstract:

Objective The aim of this study was to induce oral submucous fibrosis (OSF) in Sprague-Dawley(SD) rat models by arecoline and mechanical stimulation. Methods Two factors factorial design was used to divide 48 rats into 8 groups (n=6). Different concentrations of arecoline (0, 0.5, 2, and 8 mg·mL ^-1) and mechanical stimulation (with or without brush) were treated. After 16 weeks of treatment, the mouth opening was measured, the pathological changes of the buccal mucosa were observed, and the expressions of type Ⅲ collagen, transforming growth factor β1 (TGF-β1), and interferon-γ (IFN-γ) were detected. Results In rats with moderate and high concentrations of arecoline, typical OSF pathological features were observed in the buccal mucosa, the mouth openings were significantly reduced, and the expression levels of type Ⅲ collagen and TGF-β1 were significantly increased (P<0.05). Although mechanical stimulation can increase the three indexes of mucosa (P<0.05), no pathological change and difference in the mouth opening was observed (P>0.05). Conclusion Moderate and high concentrations of arecoline can induce OSF in SD rats, but mechanical stimulation cannot induce OSF.

Key words: arecoline, mechanical stimulation, oral submucous fibrosis, animal model

CLC Number:

R781.5

Bo Yang,Mengfan Fu,Zhangui Tang. Rat model with oral submucous fibrosis induced by arecoline and mechanical stimulation[J]. West China Journal of Stomatology, 2019, 37(3): 260-264.

Figures/Tables 7

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机械刺激	槟榔碱浓度/（mg·mL^-1）
机械刺激	0	0.5	2	8
无	A	B	C	D
有	E	F	G	H

组别	开口度/cm	Ⅲ型胶原	TGF-β1	IFN-γ
A	2.203±0.115	0.08±0.06	0.18±0.05	0.16±0.08
B	2.191±0.069	0.11±0.06	0.19±0.02	0.17±0.09
C	2.000±0.104	0.12±0.06	0.25±0.04	0.12±0.09
D	1.942±0.073	0.14±0.08	0.24±0.03	0.15±0.07
E	2.190±0.084	0.21±0.06	0.28±0.03	0.51±0.10
F	2.167±0.070	0.23±0.09	0.29±0.05	0.41±0.09
G	2.003±0.156	0.38±0.08	0.39±0.06	0.41±0.11
H	1.978±0.078	0.41±0.08	0.39±0.06	0.38±0.09

源	开口度		Ⅲ型胶原		TGF-β1		IFN-γ
源	F值	P值	F值	P值	F值	P值	F值	P值
槟榔碱浓度（a）	18.591	0.000^*	82.144	0.000^*	13.390	0.000^*	1.441	0.245
机械刺激（b）	0.002	0.965	9.244	0.000^*	87.125	0.000^*	109.539	0.000^*
槟榔碱浓度×机械刺激（a×b）	0.232	0.873	3.016	0.041^*	1.172	0.333	1.035	0.388

槟榔碱浓度（I）/	槟榔碱浓度（J）/	开口度		Ⅲ型胶原		TGF-β1
（mg·mL^-1）	（mg·mL^-1）	平均值差值（I-J）	P值	平均值差值（I-J）	P值	平均值差值（I-J）	P值
0	0.5	0.018 3	0.647	-0.026 7	0.384	-0.005 8	0.757
	2	0.195 0	0.000^*	-0.111 7	0.001^*	-0.090 0	0.000^*
	8	0.237 5	0.000^*	-0.135 0	0.000^*	-0.083 3	0.000^*
0.5	2	0.176 7	0.000^*	-0.085 0	0.008^*	-0.084 2	0.000^*
	8	0.219 2	0.000^*	-0.108 3	0.001^*	-0.077 5	0.000^*
2	8	0.042 5	0.291	-0.023 3	0.446	0.006 7	0.724