Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering

doi:10.7518/hxkq.2019.03.005

Abstract

Abstract:

Objective This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engineering. Methods Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2:1, 1:1 and 1:2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA composite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering. Results The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1:1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility. Conclusion The CMCS/OSA compound hydrogel prepared with a 1:1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.

Key words: carboxymethyl chitosan, sodium alginate, hydrogel, umbilical cord mesenchymalstem cell, cartilage tissue engineering

CLC Number:

R781.1

Yunjie Li,Binhong Teng,Yanhong Zhao,Qiang Yang,Lianyong Wang,Ying Huang. Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering[J]. West China Journal of Stomatology, 2019, 37(3): 253-259.

Figures/Tables 6

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