West China Journal of Stomatology ›› 2019, Vol. 37 ›› Issue (2): 162-167.doi: 10.7518/hxkq.2019.02.007

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Effect of sclerostin on the functions and related mechanisms of cementoblasts under mechanical stress

Siyu Bai,Yue Chen,Hongwei Dai,Lan Huang()   

  1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2018-08-20 Revised:2019-01-20 Online:2019-04-01 Published:2019-04-28
  • Contact: Lan Huang E-mail:lanhuang29@126.com
  • Supported by:
    The National Natural Science Foundation of China for Young Scholars(81300914);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016(CXTDG201602006)

Abstract:

Objective The purpose of this study is to investigate the potential effects of sclerostin (SOST) on the biological funtions and related mechanisms of cementoblasts under mechanical stress. Methods OCCM-30 cells were treated with varying doses of SOST (0, 25, 50, and 100 ng·mL -1) and were loaded with uniaxial compressive stress (2 000 μ strain with a frequency of 0.5 Hz) for six hours. Western blot was utilized to detect the expressions of β-catenin, p-smad1/5/8, and smad1/5/8 proteins. Alkaline phosphatase (ALP) activity was determined, and reverse transcription polymerase chain reaction was used to measure the expressions of runt-related transcription factor 2 (Runx-2), osteocalcin (OCN), bone sialoproteins (BSP), receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) mRNA. Results The expression of p-smad 1/5/8 was significantly downregulated with increasing SOST. β-catenin and smad1/5/8 exhibited no difference. ALP activity decreased under mechanical compressive stress with increasing SOST concentrations. Runx-2 expression was reduced with increasing SOST concentrations, and a similar trend was observed for the BSP and OCN expressions. When the SOST concentration was enhanced, RANKL expression gradually increased, whereas the expression of OPG decreased. Conclusion Under mechanical comprehensive stress, SOST can adjust the bone morphogenetic protein (BMP) /smad signal pathway. Osteosclerosis inhibits the mineralization of cementoblasts under mechanical compressive stress, which may be achieved by inhibiting the expressions of osteogenesis factors (Runx2, OCN, BSP, and others) and by promoting the ratio of cementoclast-related factors (RANKL/OPG) through BMP signal pathways.

Key words: cementoblast, mechanical stress, mineralization, sclerostin, signal pathway

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