An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells

doi:10.7518/hxkq.2018.03.003

Abstract

Abstract:

Objective This study aimed to explore the effects of concentrate growth factor extracts (CGFe) on human umbi-lical vein endothelial cells (HUVECs) in vitro. Methods Concentrate growth factor (CGF) were prepared from the peripheral blood of healthy donors, followed by CGFe. Four groups were designed based on cell culture medium, as follows: 2%CGFe, 5%CGFe, 10%CGFe, and control. The proliferation activity of HUVECs was detected by cell cycle and CCK-8 assays. The migration of HUVECs was detected by scratch assay. The mRNA expression levels of vascular endothelial growth factor (VEGF), chemokine receptor 4 (CXCR4), and platelet derived growth factor (PDGF) were examined by quantitative real time polymerase chain reaction (qRT-PCR). Results Results of CCK-8 and cell cycle assays showed that CGFe promoted the proli-feration capability of HUVECs in a dose-dependent manner, and the data had statistical significance among four groups (P<0.05). The cell migration assay indicated that CGF accelerated wound closure in a dose-dependent manner after 12 h of culture (P<0.05). The results of qRT-PCR showed that CGF upregulated the expression levels of VEGF, CXCR4, and PDGF in HUVECs. Conclusion CGFe can promote the proliferation, migration, and angiogenic differentiation of HUVECs. Thus, CGF might be an appropriate cure for dental pulp revascularization.

Key words: concentrate growth factor, human umbilical vein endothelial cells, proliferation, differentiation

CLC Number:

Jun Huan, Lei Dou, Qifang Yan, Deqin Yang. An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells[J]. West China Journal of Stomatology, 2018, 36(3): 247-251.

Figures/Tables 5

References 17

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