Effect of bone morphogenetic protein-4 overexpression on the biological activity of mouse induced pluripotent stem cells

doi:10.7518/hxkq.2018.02.014

Abstract

Abstract:

Objective This study aimed to construct the expression of bone morphogenetic protein-4 (BMP4) lentiviral vector gene and explore its influence on the biological activity of mouse induced pluripotent stem (iPS) cells. Methods iPS cell lines stably overexpressing BMP4 were constructed by lentivirus transfection (BMP4-overexpressing group). Cells without transfection served as the blank group, and cells with only vector transfection served as the empty-vector group. Cell prolifera-tion was detected by CCK8, and the expression levels of ameloblastin (AMBN), cytokeratin (CK) 14, dentin sialophospho-protein (DSPP), bone sialoprotein (BSP), and Runx2 mRNA were detected by quantitative polymerase chain reaction. Alkaline phosphatase (ALP) activity was used to detect the degree of cell differentiation. Results Compared with blank and empty-vector groups, proliferation activity and ALP activity of BMP4-overexpressing group obvious increased (P<0.05), BMP4, AMBN, CK14, DSPP, BSP, Runx2 mRNA expression also increased (P<0.05). Conclusion BMP4 can significantly promote the odontogenic differentiation of iPS.

Key words: bone morphogenetic protein-4, induced pluripotent stem cells, odontogenic differentiation, proliferation

CLC Number:

Q254

Jin Qiu, Huiyu Zhang, Li Liu, Yinghui Tan. Effect of bone morphogenetic protein-4 overexpression on the biological activity of mouse induced pluripotent stem cells[J]. West China Journal of Stomatology, 2018, 36(2): 190-193.

Figures/Tables 4

Tab 1

Fig 1

Fig 2

Tab 2

References 8

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引物名称	引物序列（5’—3’）
BMP4	上游引物：CGCCAGCCGAGCCAACAC
	下游引物：GGTCCACCTGCTCCCGAAAGA
Runx2	上游引物：CGACAGTCCCAACTTCCTGT
	下游引物：CGGTAACCACAGTCCCATCT
AMBN	上游引物：CTGTTACCAAAGGCCCTG
	下游引物：GCCATTTGTGAAAGGAGAGC
CK14	上游引物：CTCCTCTGGCTCTCAGCAGTCATC
	下游引物：AGGGACAATACAGGGGCTCTTCC
DSPP	上游引物：CTCGGAGGCTTTGAAGACATTGA
	下游引物：GCTGCAGTTCCTGGATGTGTTAGA
BSP	上游引物：AAAATGGAGACGGCGATAGTT
	下游引物：GAGTGTGGAAAGTGTGGAGTTCT
GAPDH	上游引物：GGAGATTGTTGCCATCAACGA
	下游引物：GAAGACACCAGTAGACTCCACGACA

组别	Runx2	AMBN	CK14	DSPP	BSP
空白组	1.32	2.36	1.35	2.98	2.48
空载体组	1.46	3.27	2.12	5.36	3.62
BMP4过表达组	1.79	4.56	3.89	40.28	31.90

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