Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts

doi:10.7518/hxkq.2018.02.005

Abstract

Abstract:

Objective To study the effect of the inhibitory concentration minocycline on the proliferation, differentiation, and expression of Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and osteopontin (OPN) mRNA of osteoblasts. Methods Primary osteoblasts were cultured in osteogenic induction medium containing 0, 0.1, 0.5, 1, 10 μg·mL^-1minocycline. Cell counting kit-8 was used to observe cell proliferation. ALP activity assay, alizarin red S staining, and real-time quantitative polymerase chain reaction (PCR) were used to determine cell differentiation and mineralization. Results The groups with 0.1, 0.5, 1 μg·mL^-1 minocycline promoted cell proliferation. The mRNA expression levels of ALP and Runx2 were up-regulated. Osteoblast-mediated mineralization was increased. The group with 1 μg·mL^-1 showed maximal promotion effect (P<0.05). When the concentration increased to 10 μg·mL^-1, the promoting effect began to decline, and the ALP activity and OPN expression were significantly inhibited (P<0.01). Conclusion Appropriate concentration of minocycline can promote osteoblasts proliferation, up-regulate the expression levels of Runx2, ALP and OPN, and increase the differen-tiation and mineralization of osteoblasts.

Key words: minocycline, osteoblast, alkaline phosphatase, proliferation

CLC Number:

R781.05

Huaying Shao, Yigong Zhang, Xue Yang, Qiongyue Zhang, Xiaohong Wu. Effects of inhibitory concentration minocycline on the proliferation, differentiation, and mineralization of osteoblasts[J]. West China Journal of Stomatology, 2018, 36(2): 140-145.

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