Protective effects of hydrogen-rich medium on lipopolysaccharides-induced injury in human periodontal ligament cells

doi:10.7518/hxkq.2018.02.002

Abstract

Abstract:

Objective In this study, lipopolysaccharides (LPS) was used to damage human periodontal ligament cells (hPDLCs) and consequently investigate the protective effects of hydrogen on reducing oxidative stress and cell apoptosis rate. Methods hPDLCs were isolated, and then cultured with normal medium+1 μg·mL^-1LPS or with hydrogen-rich medium+1 μg·mL^-1 LPS. Cell proliferation activity was assessed using a cell counting kit-8 (CCK-8), and lactic dehydrogenase (LDH) release was also detected. The activities of superoxide dismutase (SOD) and catalase (CAT), and the level of malonaldehyde (MDA) in supernatants were also measured. Cell apoptosis was detected by flow cytometry at 24 h after LPS stimulation. Results CCK-8 results showed that hydrogen could significantly improve hPDLCs growth and decrease cell apoptosis under LPS stimulation (P<0.05). However, no significant difference in LDH release was found between the two groups. The CAT levels significantly increased at 6 and 12 h in the hydrogen-rich medium as compared with the normal medium group (P<0.05, P<0.01, respectively). However, SOD levels were not significant different at each time point. At 6 h after LPS stimulation, the MDA levels in the cell supernatant of hydrogen-rich medium group were significantly reduced as compared with those in the normal medium group (P<0.05). Conclusion The hydrogen-rich medium can effectively improve hPDLCs proliferation activity and antioxidant capacity and reduce apoptosis and oxidative stress under LPS stimulation.

Key words: hydrogen-rich medium, lipopolysaccharides, periodontal ligament cells, oxidative damage

CLC Number:

R781.05

Min Zhou, Zuolin. Wang. Protective effects of hydrogen-rich medium on lipopolysaccharides-induced injury in human periodontal ligament cells[J]. West China Journal of Stomatology, 2018, 36(2): 123-127.

Figures/Tables 4

References 22

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