Experimental research on Arginine-gingipain A gene vaccine from Porphyromonas gingivalis that prevents peri-implantitis in Beagle dogs

doi:10.7518/hxkq.2018.01.015

Abstract

Abstract:

Objective This study aims to use Arginine-gingipain A gene vaccine (pVAX1-rgpA) to immunize adult Beagle dogs and to evaluate its effect during peri-implantitis progression and development. Methods Plasmid pVAX1-rgpA was constructed. The second and third bilateral mandible premolars of 15 adult Beagle dogs were extracted, and the implants were placed immediately. After 3 months, the animals were randomly divided into groups A, B, and C. Afterward, the animals were immunized thrice with plasmid pVAX1-rgpA, with heat-killed Porphyromonas gingivalis, or pVAX1, respectively. IgG in the serum and secretory IgA (sIgA) in saliva were quantitatively analyzed by enzyme-linked immunosorbent assay before and after 2 weeks of immunization. Peri-implantitis was induced with cotton ligatures fixed around the neck of implants. Probing depth (PD) and bleeding on probing were recorded. All animals were sacrificed after ligaturation for 6 weeks. Decalcified sections with thickness of 50 μm were prepared and dyed with methylene blue to observe the bone phenotype around implants. Results Levels of serum IgG and sIgA in saliva were higher in groups A and B after immunization than before the process (P<0.05) and higher than those in group C (P<0.05). However, no difference was observed between groups A and B (P>0.05). At 4 and 6 weeks after ligaturation, PD of the ligatured side in group C was higher than that in groups A and B (P<0.05). On the other hand, no difference was identified between groups A and B (P>0.05). Bone loss in group A was sig-nificantly lower than that of the other groups (P<0.05). Abundant inflammatory cells and bacteria were present in the bone loss area around the implants in the three groups, as identified through hard tissue section observation. However, group C presented the most number of inflammatory cells and bacteria in the bone loss area around the implants. Conclusion IgG and sIgA can be generated by immunity with rgpA DNA vaccine, which can significantly slow down bone loss during expe-rimental peri-implantitis in dogs.

Key words: gene vaccine, dental implant, peri-implantitis, Arginine-gingipains, Porphyromonas gingivalis

CLC Number:

R783

Chuanhua Li, Zhifeng Wang, Lina Zhu, Xin Fan, Jing Lan. Experimental research on Arginine-gingipain A gene vaccine from Porphyromonas gingivalis that prevents peri-implantitis in Beagle dogs[J]. West China Journal of Stomatology, 2018, 36(1): 76-81.

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组别	2周	4周	6周
A₁	2.13±0.09	3.47±0.12	4.32±0.73
A₂	0.66±0.18	0.82±0.26	1.02±0.37
B₁	2.51±0.11	3.78±0.11	4.86±0.36
B₂	0.72±0.23	0.89±0.34	1.37±0.48
C₁	3.58±0.99	5.12±0.09	5.37±0.41
C₂	0.79±0.28	0.97±0.27	1.46±0.52

组别	免疫前		免疫后
组别	sIgA	IgG	sIgA	IgG
A	2.57±0.09	2.37±0.12	4.32±0.73*	5.00±0.42*
B	2.51±0.11	2.41±0.12	4.15±0.36*	2.39±0.28*
C	2.48±0.99	2.33±0.09	2.27±0.41	2.53±0.46