Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells

doi:10.7518/hxkq.2018.01.005

Abstract

Abstract:

Objective This study aims to investigate the effect of neurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs). Methods hDFCs were isolated and cultured in vitro. Immunocytochemical staining was used to identify the origin of hDFCs. The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay. The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis. The difference in the number of mineralized nodules was detected using alizarin red staining. Results Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells. NT-3 exerted no evident effect on hDFCs proliferation. The ALP activity and the BMP-2 and OCN mRNA expression levels of hDFCs were signiﬁcantly improved under treatment with different NT-3 concentrations (25, 50, and 100 ng·mL^-1) compared with those in the control group. BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL^-1. The number of mine-ralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL^-1 NT-3. Conclusion Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.

Key words: neurotrophin 3, human dental follicle cells, osteogenic differentiation

CLC Number:

R780.2

Wei Lei, Yue Ma, Aishu Ren. Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells[J]. West China Journal of Stomatology, 2018, 36(1): 23-28.

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