Correlation between interleukin-10 polymorphisms and susceptibility to chronic periodontitis among Uygur adults in the Moyu area

doi:10.7518/hxkq.2017.05.014

Abstract

Abstract:

Objective This study aims to investigate the association between interleukin (IL)-10-597 (C/A) single-nucleotide polymorphisms and chronic periodontitis of Moyu Uygur population in Xinjiang Uygur Autonomous Region of China. Methods In accordance with the inclusion and exclusion criteria, the buccal swabs of 300 subjects were randomly selected from the epidemiological investigation of Uygur adults in Moyu county on April and May 2013. The study was conducted on a healthy control group, a mild chronic periodontitis group, and a moderate-to-severe chronic periodontitis group, with each comprising 100 samples. The IL-10-597(C/A) site in the promoter sequences was analyzed using the tetra-primer amplification refractory mutation system-polymerase chain reaction method to test the genotype and allele distributions. Statistical analysis was performed using Chi-squared test and ordinal classification Logistic regression analysis. Results The genotype and allelefrequencies of the IL-10-597(C/A) site in the healthy control group, mild chronic periodontitis group, and moderate-to-severe chronic periodontitis group exhibited no significant difference (P>0.05). The age of all the samples was associated with chronic periodontitis. The risk of chronic periodontitis in the people of 55-65 years old was 25 times in the people under the age of 35 (OR=25.56, P<0.001). Conclusion The IL-10-597 (C/A) single-nucleotide polymorphisms in the gene promoter are not associated with chronic periodontitis in Uygur adult population.

Key words: interleukin-10, chronic periodontitis, single nucleotide polymorphisms

CLC Number:

R781.4

Yuhui Zhang, Ping Huang, Jing Lin, Jin Zhao. Correlation between interleukin-10 polymorphisms and susceptibility to chronic periodontitis among Uygur adults in the Moyu area[J]. West China Journal of Stomatology, 2017, 35(5): 514-519.

Figures/Tables 5

Tab 1

Fig 1

Tab 2

Tab 3

Tab 4

References 24

[1]	Gaffen SL, Hajishengallis G.A new inflammatory cytokine on the block: re-thinking periodontal disease and the Th1/Th2 paradigm in the context of Th17 cells and IL-17[J]. J Dent Res, 2008, 87(9):817-828.
[2]	O’Garra A, Barrat FJ, Castro AG, et al. Strategies for use of IL-10 or its antagonists in human disease[J]. Immunol Rev, 2008, 223:114-131.
[3]	Loos BG, John RP, Laine ML.Identification of genetic risk factors for periodontitis and possible mechanisms of action[J]. J Clin Periodontol, 2005, 32(Suppl 6):159-179.
[4]	Yamazaki K, Tabeta K, Nakajima T, et al.Interleukin-10 gene promoter polymorphism in Japanese patients with adult and early-onset periodontitis[J]. J Clin Periodontol, 2001, 28(9):828-832.
[5]	Edwards-Smith CJ, Jonsson JR, Purdie DM, et al.Interleukin-10 promoter polymorphism predicts initial response of chro-nic hepatitis C to interferon alfa[J]. Hepatology, 1999, 30(2):526-530.
[6]	桑晓红, 王顺, 刘健, 等. 新疆墨玉县维吾尔族成人牙周炎与慢性肾脏病患病率的流行病学研究[J]. 中华肾脏病杂志, 2010, 26(7):510-515.
	Sang XH, Wang S, Liu J, et al.Epidemiologic study on the relationship between periodontitis and chronic kidney disease among Uygur adults in Moyu county, Xinjiang[J]. Chin J Nephrol, 2010, 26(7):510-515.
[7]	王东麟, 吴龙, 林静, 等. 新疆墨玉地区维吾尔族成人中、重度慢性牙周炎与雌激素受体基因多态性的相关性研究[J]. 医学研究生学报, 2015, 28(3):280-285.
	Wang DL, Wu L, Lin J, et al.Correlation of estrogen recep-tor polymorphisms with the susceptibility to moderate to severe chronic peri-odontitis among Uygur adults in the Moyu area of Xinjiang[J]. J Med Postgraduat, 2015, 28(3):280-285.
[8]	吴龙, 林静, 王东麟, 等. 新疆墨玉县维吾尔族成人慢性牙周炎与维生素D受体基因多态性的相关性研究[J]. 口腔医学研究, 2015, 31(10):995-999.
	Wu L, Lin J, Wang DL, et al.Association between chronic periodontitis and vitamin D receptor gene polymorphisms among Uygur adults in Moyu county of Xinjiang[J]. J Oral Sci Res, 2015, 31(10):995-999.
[9]	齐小秋. 第三次全国口腔健康流行病学调查报告[M]. 北京: 人民卫生出版社, 2008:98-99.
	Qi XQ.The third national oral health epidemiological sur-vey report[M]. Beijing: People’s Medical Publishing House, 2008:98-99.
[10]	Muthukumar S, Suresh R.Community periodontal index of treatment needs index: an indicator of anaerobic periodontal infection[J]. Indian J Dent Res, 2009, 20(4):423-425.
[11]	曹采方. 临床牙周病学[M]. 北京: 北京大学医学出版社, 2006:172.
	Cao CF.Clinical periodontal disease[M]. Beijing: Peking University Medical Press, 2006:172.
[12]	Albuquerque CM, Cortinhas AJ, Morinha FJ, et al.Associa-tion of the IL-10 polymorphisms and periodontitis: a meta-analysis[J]. Mol Biol Rep, 2012, 39(10):9319-9329.
[13]	Löe H, Anerud A, Boysen H, et al.Natural history of perio-dontal disease in man. Rapid, moderate and no loss of attach-ment in Sri Lankan laborers 14 to 46 years of age[J]. J Clin Periodontol, 1986, 13(5):431-445.
[14]	Tworek D, Kuna P.The role of interleukin 10 in allergic inflammation[J]. Pol Merkur Lekarski, 2005, 18(104):125-128.
[15]	Górska R, Gregorek H, Kowalski J, et al.Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis[J]. J Clin Periodontol, 2003, 30(12):1046-1052.
[16]	Gonzales JR, Michel J, Diete A, et al.Analysis of genetic polymorphisms at the interleukin-10 loci in aggressive and chronic periodontitis[J]. J Clin Periodontol, 2002, 29(9):816-822.
[17]	Al-Rasheed A, Scheerens H, Rennick DM, et al.Accelerated alveolar bone loss in mice lacking interleukin-10[J]. J Dent Res, 2003, 82(8):632-635.
[18]	孟凡琦. 墨玉县农村中老年人口腔健康状况及就医观念行为调查分析[D]. 乌鲁木齐: 新疆医科大学, 2015:8-16.
	Meng FQ.Investigational analysis of oral health status, dental treatment behavior and concepts among the elderly in rural area of Karakax county in Xinjiang[D]. Urumqi: Xinjiang Medical University, 2015:8-16.
[19]	侯雪燕. 墨玉县维吾尔族成人牙周炎现状及牙周炎与全身因素相关性的流行病学研究[D]. 乌鲁木齐: 新疆医科大学, 2015:10-16.
	Hou XY.Epidemiological study of periodontal condition and relationship between periodontitis and systemic factors of Uygur people in Moyu county[D]. Urumqi: Xinjiang Medical University, 2015:10-16.
[20]	唐燕, 章锦才, 张蕴惠. IL-10-819位点基因多态性与汉族人群重度慢性牙周炎易感性相关性的研究[J]. 广东牙病防治, 2004, 12(1):12-14.
	Tang Y, Zhang JC, Zhang YH.Association of IL-10-819 polymorphism with the susceptibility to severe chronic perio-dontitis in Chinese Han nationality[J]. J Dent Prev Treat, 2004, 12(1):12-14.
[21]	Jaradat SM, Ababneh KT, Jaradat SA, et al.Association of interleukin-10 gene promoter polymorphisms with chronic and aggressive periodontitis[J]. Oral Dis, 2012, 18(3):271-279.
[22]	Scarel-Caminaga RM, Trevilatto PC, Souza AP, et al.Inter-leukin 10 gene promoter polymorphisms are associated with chronic periodontitis[J]. J Clin Periodontol, 2004, 31(6):
	443-448.
[23]	Sumer AP, Kara N, Keles GC, et al.Association of interleu-kin-10 gene polymorphisms with severe generalized chronic periodontitis[J]. J Periodontol, 2007, 78(3):493-497.
[24]	Hu KF, Huang KC, Ho YP, et al.Interleukin-10 (-592C/A) and interleukin-12B (+16974A/C) gene polymorphisms and the interleukin-10 ATA haplotype are associated with perio-dontitis in a Taiwanese population[J]. J Periodont Res, 2009, 44(3):378-385.

项目分类		分组
项目分类		健康对照	轻度CP	中重度CP
性别^1）	男	34	50	60
	女	66	50	40
年龄/岁^2）		34.6±11.0	40.3±11.3	53.0±10.0
CAL/mm		NA	1.76±0.55	4.51±1.44
PD/mm^3）		1.26±0.38	2.09±0.50	2.47±0.64

分组	AA/%		AC/%		CC/%		卡方值	P值
分组	观察值	期望值	观察值	期望值	观察值	期望值	卡方值	P值
健康对照	19/19.0	20.0/20.2	52/52.0	50.0/49.5	29/29.0	30.0/30.2	0.820	0.960
轻度CP	25/25.0	23.0/23.3	46/46.5	49.5/50.0	28/28.0	26.0/26.5	0.324	0.850
中重度CP	25/25.0	24.5/24.5	49/49.0	50.0/50.0	26/26.0	25.5/25.5	0.005	0.997

组别	基因型			等位基因n/%
组别	AA	AC	CC	A	C
健康对照	19	52	29	90/45.0	110/55.0
轻度CP	25	46	28	96/48.5	102/51.5
中重度CP	25	49	26	99/49.5	101/50.5
卡方值	1.571			0.893
P值	0.814			0.640

项目分类		OR值	95%CI	P值
性别	男	0.44	0.28~1.41	0.001*
	女	1.00	-	-
年龄/岁	<35	1.00	-	-
	35~	1.80	0.86~3.62	0.101
	45~	7.90	3.93~15.89	<0.001*
	55~65	25.56	12.29~53.14	<0.001*
基因型	AA	0.78	0.41~1.49	0.460
	AC	0.85	0.50~1.47	0.569
	CC	1.00	-	-