Inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the prolifera-tion of salivary adenoid cystic carcinoma ACC-83 cells

doi:10.7518/hxkq.2017.03.016

Abstract

Abstract:

Objective To investigate the inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the proliferation of salivary adenoid cystic carcinoma ACC-83 cells. Methods In vitro cell culture was performed. The proliferation of ACC-83 cells treated with different concentrations (5, 10, 20, 40, 60, 80, 100 μg·mL^-1) of total saponins from Paris polyphylla var. yunnanensis was observed using CCK-8 assay. Meanwhile, the apoptosis of ACC-83 cells treated with different concentrations (25, 50, 100 μg·mL^-1) of the total saponins was observed using flow cytometry. The expression levels of macrophage migration inhibitory factor (MIF) and CD74 were measured using Western blot and reverse transcription-polymerase chain reaction. Results The total saponins from Paris polyphylla var. yunnanensis induced apoptosis and expressed dose-effect relationship. ACC-83 cells expressed MIF and CD74, and the total saponins suppressed MIF and CD74 expression in ACC-83 cells. Conclusion The total saponins from Paris polyphylla var. yunnanensis can significantly inhibit the proliferation, suppress MIF and CD74 expression, and promote apoptosis in ACC-83 cells. This study provides a theoretical basis for the treatment of salivary adenoid cystic carcinoma using Paris polyphylla var. yunnanensis.

Key words: total saponins from Paris polyphylla var. yunnanensis, salivary adenoid cystic carcinoma, macro-phage migration inhibitory factor

CLC Number:

R 730.52

Qiumin He, Biao Xu, Weihong Wang, Chongyun Bao, Shao-wei Hu. Inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the prolifera-tion of salivary adenoid cystic carcinoma ACC-83 cells[J]. West China Journal of Stomatology, 2017, 35(3): 317-321.

Figures/Tables 4

References 17

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药物质量浓度/（μg·mL^-1）	OD值	抑制率/%
阴性对照	1.646 33±0.011 2	-
5	1.546 11±0.026 6	6.721±2.351
10	1.438 67±0.047 7	14.264±2.267
20	1.174 73±0.004 4	30.807±6.279
40	0.979 28±0.043 1	43.792±3.343
60	0.697 69±0.021 6	62.295±2.801
80	0.535 33±0.021 2	73.429±0.402
100	0.318 42±0.003 6	87.407±2.333