West China Journal of Stomatology ›› 2015, Vol. 33 ›› Issue (6): 622-626.doi: 10.7518/hxkq.2015.06.015

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Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells

Tang Cuizhu1,2, Wen Yong1,2, Gu Weiting3, Zhang Bing1, Zhang Yunpeng1, Ji Yawen1, Xu Xin1,2   

  1. 1. School of Stomatology, Shandong University, Jinan 250012, China; 2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan250012, China; 3. Dept. of Gynaecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, China)
  • Received:2015-05-04 Revised:2015-09-07 Online:2015-12-01 Published:2015-12-01

Abstract: Objective To investigate the effects of small interfering RNA (siRNA) targeting YAP on the proliferationand apoptosis of human periodontal ligament stem cells (hPDLSCs). Methods Synthesized sequences of siRNA were transfectedinto hPDLSCs by LipofectamineTM 2000. The expression of YAP was identified by using real-time quantitative reversetranscription-polymerase chain reaction (RT-PCR) and Western blot analysis. Proliferation activity was detected by usingcell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate were detected by using flow cytometry. Resultswere analyzed by using SPSS 19.0, and P<0.05 was considered statistically significant. Results Expression of YAP mRNAand protein were significantly downregulated after 48 h of transfection (P<0.001). No obvious difference was found in theexpression levels of YAP protein between 48 and 72 h, thus indicating that siRNA could inhibit the expression of YAPpersistently and effectively. Proliferation activity was inhibited, and apoptosis rate was increased. Cell cycle was changedas the proportion of G1 and S phases increased (P<0.01) and G2 phase decreased (P<0.05). Conclusion Knocking down YAP gene by siRNA could inhibit proliferation activity,induce apoptosis, and change the cell cycle of hPDLSCs.Thus, YAP could regulate the proliferation and apoptosisof hPDLSCs.

Key words: small interfering RNA, YAP gene, humanperiodontal ligament stem cells, proli-feration, apoptosis

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