Abstract:

Objective  To investigate the synergistic effect of transforming growth factor-β1(TGF-β1)and platelet-derived growth factor-BB(PDGF-BB) on the expression of integrin β3 in periodontal membrane of rat orthodontic tooth. Methods  An orthodontic tooth movement model was established. Up to 32 experimental rats were randomly divided into four groups according to a random number table. The four groups were injected with 1%PBS, TGF-β1(5 ng), PDGF-BB (10 ng), and combined TGF-β1(5 ng) and PDGF-BB (10 ng) in the buccal submucosal, respectively. The volume injected in each group was 0.1 mL. The animals were then sacrificed on the 10th day. The left maxillary first molar and periodontal tissue were taken. Different expressions of integrin β3 were detected in periodontal tissues through immunohistochemistry. Mean optical density (OD) values of the positive fields were examined. The data obtained were analyzed through ANOVA. The data followed normal distribution, and were compared via t-test. Results  Compared with the control groups, the expression of integrin β3 was higher in the experimental groupin tension sides (P<0.01). Significant differences in tension sides between the single-injection groups and the combined group were observed (P<0.01). Compared with the control groups, the expression of integrin β3 was higher in the experimental group in compression sides (P<0.05). In addition, there was no significant differences in compression sides between the single-injection groups and the combined group (P>0.05). Conclusion  In terms of local regulatory factors, TGF-β1 combined with PDGF-BB enhance the expression of integrinβ3 in the periodontal membrane and accelerate periodontal remodeling.The synergistic effect of the twogrowth factorsis better than the single growth factor.

Key words: transforming growth factor-β1, platelet-derived growth factor-BB, integrin β3, periodontal tissue of orthodontic tooth