West China Journal of Stomatology

Previous Articles     Next Articles

Differential expression profiles of microRNAs/mRNA and docking study in oral squamous cell carcinoma

 Liu Siyu1,2, Xie Long1,2, Qi Bing3, Ma Changyan4, Sang Lei1,2, Li Hongwei1,2   

  1. 1.Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China;2.Dept. of Oral and Maxillofacial Surgery, The Stomatology Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China; 3. Dept. of Oral Pathology, The Stomatology Hospital Affiliated to Nanjing Medical Uni-versity, Nanjing 210029, China; 4. Dept. of Developmental Genetics, Nanjing Medical University, Nanjing 210029, China
  • Online:2014-08-01 Published:2014-08-01

Abstract:

 Objective  To construct and analyze the differential expression profiles of microRNAs (miRNA) and mRNA in oral squamous cell carcinoma(OSCC).   To explore the miRNA and mRNA of OSCC development and progression. Methods  The differential expression profiles of miRNA and mRNA were built by high-throughput deep sequencing technology. Using Gene Ontology (GO) enrichment analysis, the roles of differentially expressed miRNA and mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis were analyzed. Results  Seventy-seven differentially expressed miRNA and 1 298 differentially expressed mRNAs were identified in OSCC. GO analysis showed that 73 miRNA had found target mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis of OSCC. Moreover, a miRNA could regulate multiple mRNA. Conclusion The differential expression profiles of miRNA and mRNA have close relationship with the development and progression of OSCC.

Key words: oral squamous cell carcinoma, microRNAs, mRNA, high-throughput deep sequencing