West China Journal of Stomatology

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Effect on secretory function of rat submandibular glands caused by ischemia reperfusion

Xiao Meng1,2, Liu Shaohua1, Liu Yunsheng1,2, Wei Fengcai1, Shi Liang1   

  1. 1. Dept. of Oral and Maxillofacial Surgery, Qilu Hospital, Shandong Univer-sity; Institute of Dental Medicine, Shandong University, Jinan 250012, China; 2. School of Stomatology, Shandong Univer-sity; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China
  • Online:2014-06-01 Published:2014-06-01

Abstract:

Objective  To investigate the injury stress responses caused by ischemia reperfusion and its effects on the sa-livary secretory function of rat submandibular glands. Methods  An in situ ischemia reperfusion experimental model of rat submandibular glands was developed. The rat submandibular glands were subjected to 90 min of ischemia without dener-vation followed by reperfusion for 1, 12, 24, and 72 h. Salivary secretion, histological changes, reactive oxygen species (ROS) levels, and cellular apoptosis of the involved submandibular glands were detected after reperfusion. Results  The secretory function of the glands decreased at 1 and 12 h, and the saliva secretion gradually had the same value as that of the control sample 72 h after reperfusion. Increasing inflammatory cells infiltration, cellular atrophy, and tissue edema were observed especially after reperfusion for 12 h. The level of ROS and the number of apoptotic cells exhibited the same tendency, and higher ROS levels and more apoptosis cells 1 and 12 h after reperfusion were observed. Conclusion  Our study suggests that ischemia reperfusion can cause a series of injury stress responses in submandibular glands, which might have an important function in the early phase dysfunction of transplanted submandibular glands.

Key words: ischemia reperfusion, injury-stress responses, secretory function, submandibular gland transfer