Abstract:

Objective   To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts. Methods   Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen?Ⅰ?(Col?Ⅰ) mRNA levels were examined by real-time quantitative poly-merase chain reaction. OCN protein levels were determined by Western blot. Results   After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue (P<0.05). After 7 d of culture, OCN, Runx2, and Col?Ⅰ mRNA expression decreased (P<0.05). The OCN protein levels also decreased (P<0.05). Conclusion   Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.

Key words: peri-implantitis, osteoblast, biological function