Abstract:

Objective To investigate the relationship between c-fos gene and filamentous actin（F-actin） in MG-63 osteoblasts under cyclic tensile stress. Methods MG-63 osteoblasts were subjected to cyclic tensile strss（0.5 Hz, 2 000 μstrain） for 3, 6, and 12 h. The changes of c-fos gene were investigated by fluorescent quantitation polymerase chain reaction. Then the best loading time group was screened as the experimental group compared with 0 h group. The changes of F-actin and c-fos were investigated with or without cytochalasin D treatment. Results Cyclic tensile stress induced high expression of c-fos mRNA, and peaked at 3 h. After loading, F-actin had a structure reorganization, but had no change in expression. After cytochalasin D treatment, the formation of stress fibers and the fluorescence intensity of F-actin cytoskeleton significantly reduced, meanwhile the c-fos mRNA expression was inhibited. Conclusion After loading, there is only structure reorganization for F-actin, and the expression has not any change. That means the remodeling F-actin is the existing one. F-actin reorganization is an important part in c-fos gene expression induced by stress.

Key words: cyclic tensile stress, osteoblasts, c-fos gene, filamentous actin