Abstract:

Objective To detect the biological influence to human tongue squamous cell carcinoma（TSCC） cells of micro ribonucleic acid-21（miR-21）. Methods Referring to mature miR-21 sequence, the sense and antisense oligonucleotide（sense-miR-21 and AS-miR-21） modified by 2’O-Me were designed to transfect into TSCC cells（Tca8113 and high metastasis cells） by liposome transfection technology, in order to establish an in vitro TSCC cell model. The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polymerase chain reaction（real-time PCR）. The changes of cell proliferation, cell cycle, cell early apoptosis, cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium（MTT）, flow cytometry,  Annexin Ⅴ cell early apoptosis assay, scratch assay and Transwell assay, to check AS-miR-21’s effect on the biological
characteristics of human TSCC cell lines. Results For the TSCC cells, the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation, promoted cell apoptosis, and inhibited the capability of cell’s migration and invasion. Conclusion The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells, and miR-21 can affect biological behavior of TSCC cells.

Key words: tongue squamous cell carcinoma, micro ribonucleic acid-21, antisense-micro ribonucleic acid-21 oligonucleotide, biological effect