West China Journal of Stomatology

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The correlation analysis on Enterococcus faecalis biofilm and clinical manifestation in retreatment of root canal

Zheng Jianbo1,2, Wang Lina1, Song Qiyi1,2, Li Jiyao3, Niu Weidong1   

  1. 1. Dept. of Oral Medicine, School of Stomatology, Dalian Medical University, Dalian 116044, China; 2. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 3. Dept. of Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2012-02-25 Revised:2012-02-25 Online:2012-02-01 Published:2012-02-01
  • Contact: Niu Weidong,Tel:0411-86110399
  • About author:Zheng Jianbo(1983—),女,辽宁人,硕士

Abstract:

Objective To detect the ability of Enterococcus faecalis(E.faecalis) biofilm formation and explore the relationship between E.faecalis biofilm formation ability and clinical manifestation. Methods 96 well plate with the establishment of 53 E.faecalis in vitro biofilm model, combined with crystal violet staining, was used to test the biofilm formation ability of the clinical isolates E.faecalis and analyze the relationship between biofilm formation capacity and clinical manifestation. Results In total 53 E.faecalis strains, 40 strains(75.47%) had biofilm forming ability. Statistical analysis revealed that the capacities of biofilm formation between E.faecalis isolated from with fistula and without fistula was significantly different(P<0.05). Conclusion In the retreatment of root canal, the ability of biofilm formation of E.faecalis separated from the teeth without fistula is better than those separated from the teeth with fistula.

Key words: Enterococcus faecalis, root canal retreatment, biofilm