West China Journal of Stomatology

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Influence of epidermal growth factor receptor monoclony antibody MAb225 on DNA repair of tongue carcinoma cell after radiation

Wang Mingguo1, Wang Zhonghe2   

  1. 1. Dept. of Stomatology, The Affiliated Jinan Central ospital of Shandong University, Jinan 250013, China; 2. Dept. of Oral and Maxillofacial Surgery, The Ninth People’s ospital, School of Stomatology, Shanghai Jiao Tong University, Shanghai 200011, China
  • Received:2011-12-25 Revised:2011-12-25 Online:2011-12-20 Published:2011-12-20
  • Contact: Wang Mingguo，Tel：0531-85695171

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Abstract:

Objective The objective of this study is to investigate the effect of epidermal growth factor receptor （EGFR） monoclonal antibody MAb225 on repair of DNA double strand break（DNA-DSB） after radiation in tongue squamous cell carcinoma cell. Methods The single cell gel electrophoresis（SCGE） was performed to estimate the repair of DNA-DSB induced by radiation in human tongue carcinoma cells Tca8113 treated with or without MAb225. Expression of Ku70 and Ku80 were detected by semiquantitative reverse transcription polymerase chain reaction and Western bolt. Results Comet tail moment of MAb225 treated cell was significantly higher than untreated cell（P<0.05）. The expression of Ku70 and Ku80 were inhibited by MAb225. Conclusion MAb225 can inhibit repair of DNA-DSB induced by down-regulated expression of Ku70 and Ku80.

Key words: radiation, tongue carcinoma, DNA, double strand break, repair

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