Abstract:

Objective To investigate the morphology and proliferation effects of adenovirus containing bone morphogenetic protein-2（BMP-2） on tongue cancer Tca8113 cells. Methods Tca8113 cells were transfected with the Ad- BMP-2 of 0, 50, 100 multiplicity of infection（MOI） respectively. Inverted fluorescence microscope was used to evaluate the morphological changes of these cells. Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells. Methyl thiazolyl tetrazolium（MTT） assay was performed to monitor the proliferative activity of the infected Tca8113 cells and then the growth curve was made. Results The transfection efficiency reached  the highest when the MOI was 100. Moreover, the expression of BMP-2 was detected in Tca8113 cells by Western blot. There were no obvious morphological changes of the Tca8113 cells before and after transfection. And the proliferation of  ransfected Tca8113 cells decreased compared with control. Conclusion Ad-BMP-2 gene can inhibit the proliferation of Tca8113 cells in vitro.

Key words: bone morphogenetic protein-2, adenovirus, gene transfection, tongue cancer cells