Abstract:

Objective To explore the effects and feasibility of the repairing alveolar bone by combination of bone marrow stromal cells（BMSCs） and fibrin glue（FG）. Methods BMSCs were obtained from the sucking mouse and then separated, cultured to the second generation to get ready for use. The available cells were combined with FG and then immediately implanted into the defect that had been established in one lateral maxilla of 30 SD rats. At the same time pure FG without BMSCs was implanted into the other side. The rats were killed at the 3rd and 6th week after operation. The hematoxylin-eosin staining and Micro-CT were used to observe and evaluate the osteogenesis. Results New bone trabecula formed at experiment side of the rats at 3rd week. The repairing seemed better after 6th week of operation than the control side. There were only fibrous tissues in the control side or none. Conclusion This experiment showed a feasible approach of repairing the defects in alveolar bone of the rats.

Key words: bone marrow stromal cells, fibrin glue, tissue engineering, alveolar bone defect