Abstract:

Objective To investigate the effect of celecoxib on adhesion, invasion, migration and matrix metalloproteinase- 2（MMP-2） expression of tongue squamous cell carcinoma cell line Tca8113 cells. Methods Following 24 h incubation with celecoxib, the Tca8113 cells were detected for cell adhesion and migration using cell adhesion as-say and Boyden chamber invasion assay. The expression of cyclooxygenase-2（Cox-2） protein in Tca8113 cells was detected with SP immunohistochemistry staining. The MMP -2 level in supernatant was detected with enzyme -linked immunosorbent assay. Results The adhesion and Boyden chamber invasion assays showed that, after treatment with celecoxib, the ability of adhesion and migration of Tca8113 cells was significantly inhibited. Celecoxib could decrease the expression of Cox-2 protein in Tca8113 cell and decrease the MMP-2 level in supernatant. Conclusion Cox-2 inhibitor celecoxib can significantly inhibit the adhesion and migration of Tca8113 cells. The inhibitory effect on adhesion and migration may be correlative with its effect on decrese of Cox -2 protein expression and secretion of MMP-2 in Tca8113 cells.

Key words: cyclooxygenase-2, squamous cell carcinoma, invasion, matrix metalloproteinase-2