Abstract:

Objective To study the effect of basic fibroblast growth factor on the mRNA expression of β1 integrin subunit by periodontal ligament fibroblasts in culture; to discuss the effect of basic fibroblast growth factor in periodontal regeneration. Methods Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth factor（0.1, 1.0, 10.0 ng·mL-1） for 24, 48, 72 h respectively, and then mRNA expression of β1 integrin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction. Results Basic fibroblast growth factor enhanced the mRNA expression of β1 integrin subunit, and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng·mL-1 at 24, 48, 72 h respectively; the mRNA expression of β1 integrin subunit at 72 h was higher than that at 24, 48 h. Conclusion Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts′ adhesion and may be one of important factors which participate in the periodontal regeneration.

Key words: basic fibroblast growth factor, β1 integrin subunit, periodontal ligament fibroblast, periodontal regeneration