Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells

doi:10.3969/j.issn.1000-1182.2009.05.018

West China Journal of Stomatology

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Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells

JIANG Jun -qiang1, DING Yi2, LI Xiao -yu3, CAI Wei1, WANG Zhong -chao1

1. Dept. of Oral Medicine, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China; 2. Dept. of Periodontology, West China College of Stomatology, Sichuan University, Chengdu 610041, China; 3. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China

Received:2009-10-25 Revised:2009-10-25 Online:2009-10-20 Published:2009-10-20
Contact: DING Yi，Tel：028-61153007

Abstract

Abstract:

Objective To evaluate the biological effects of Drynaria fortunei naringin on the proliferation and alkaline phosphatase（ALP） activity of human periodontal ligament cells（hPDLC）. Methods hPDLC were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. 3-（4, 5-Dimethylthiazol-2- yl）-2, 5-diphenyltetrazolium bromide（MTT） method was used to observe the proliferation of hPDLC with the effects of different concentrations of Drynaria fortunei naringin at difference times. The method recommended by International Federation of Clinical Chemistry and Laboratory Medicine（IFCC） was adopted to investigate the effect of Drynaria fortune naringin on the alkaline phosphatase（ALP） activity of hPDLC. At the same time, we used bright blue method to detect the contents of protein in each sample. Then ALP activity in per milligram protein was accounted. Results Significant proliferative promotion to hPDLC by Drynaria fortunei naringin could be observed at the dose of 10, 1, 0.1 mg·L-1. Significant ALP activity of hPDLC promotion of Drynaria fortune naringin could be observed at the dose of 100, 10, 1, 0.1 mg·L-1 And the dose of 1 mg·L-1 Drynaria fortune naringin had greatest promotion on ALP activity of hPDLC. Conclusion Drynaria fortune naringin might significantly promote the proliferation and increase the ALP activity of hPDLC.

Key words: Drynaria fortunei naringin, periodontal ligament cells, proliferation, alkaline phosphatase activity

JIANG Jun -qiang1, DING Yi2, LI Xiao -yu3, CAI Wei1, WANG Zhong -chao1. Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2009.05.018.

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Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells

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