丝素蛋白多孔支架用于口腔软组织增厚的初步研究

doi:10.7518/hxkq.2022.05.003

华西口腔医学杂志 ›› 2022, Vol. 40 ›› Issue (5): 513-521.doi: 10.7518/hxkq.2022.05.003

丝素蛋白多孔支架用于口腔软组织增厚的初步研究

李德雄¹^,²(), 曹润源², 陈江¹^,²()

^1.福建医科大学附属口腔医院，福州 350000
^2.福建医科大学口腔医学院口腔颅面种植研究中心，福州 350002

收稿日期:2022-01-26 修回日期:2022-07-05 出版日期:2022-10-01 发布日期:2022-10-17
通讯作者: 陈江 E-mail:li331200@outlook.com;jiangchen@fjmu.edu.cn
作者简介:李德雄，讲师，博士，E-mail：li331200@outlook.com
基金资助:
国家自然科学基金(81771126);福建医科大学启航基金(2021QH1134)

Preliminary study of silk fibroin porous scaffold for oral soft-tissue thickening

Li Dexiong¹^,²(), Cao Runyuan², Chen Jiang¹^,²()

^1.School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China
^2.Institute of Stomatology & Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China

Received:2022-01-26 Revised:2022-07-05 Online:2022-10-01 Published:2022-10-17
Contact: Chen Jiang E-mail:li331200@outlook.com;jiangchen@fjmu.edu.cn
Supported by:
The National Natural Science Foundation of China(81771126);Startup Fund for Scientific Research, Fujian Medical University(2021QH1134);Correspondence: Chen Jiang, E-mail: jiangchen@fjmu.edu.cn

摘要/Abstract

摘要：

目的通过体内实验探究3种不同浓度的丝素蛋白多孔支架在口腔软组织增厚应用中的可行性。方法采用冷冻干燥及甲醇增强法制备3种不同浓度：质量分数为1%（SF1组）、3%（SF3组）、5%（SF5组）的丝素蛋白支架，通过扫描电子显微镜（SEM）、傅里叶转换红外光谱（FTIR）、X射线衍射（XRD）、热重分析（DSC）对支架进行表征分析，并测定各组支架的孔径、孔隙率及体外降解速率。将3组支架材料（实验侧）与胶原基质（对照侧）分别植入新西兰大白兔两侧口腔黏膜下，比较其术前、术后3个月黏膜厚度的变化，通过组织苏木精-伊红（HE）染色法、Masson染色法对比观察各组材料的体内代谢及再生效果。结果 SEM显示：3组支架材料都是相互交联的多孔结构；XRD及FTIR表明：3组支架均以较稳定的SilkⅡ型结构为主，在体外降解较慢；其中SF3组的孔径最大（133.40 μm±22.85 μm），孔隙率适中（90.05%±6.68%）。体内实验结果表明：除了SF1组因空间维持不足导致增厚效果类似于对照侧以外，SF3及SF5组的空间维持稳定、增厚效果明显优于对照侧；但不同于SF5组诱发了明显的炎症，SF3组体内降解较好，纤维及血管新生较多，炎症反应较少。结论丝素蛋白支架可应用于有效增厚软组织，其中以SF3组（质量分数3%）的丝素蛋白支架的理化性能、组织相容性及黏膜增厚效果最佳。

关键词: 支架, 丝素蛋白, 软组织增厚, 动物实验

Abstract:

Objective This study aimed to investigate the feasibility of three different concentrations of silk-fibroin porous scaffolds applied to oral soft-tissue thickening in vivo. Methods Silk-fibroin scaffolds with three different concentrations (1 wt%, 3 wt%, and 5 wt%; denoted as SF1, SF3, and SF5, respectively) were prepared by freeze drying and methanol enhancement. The scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and thermogravimetric analysis. Pore size, porosity, and in vitro degradation rate were also evaluated. The three groups of scaffold materials (the experimental sides) and the collagen matrix (the control side) were implanted into the oral mucosa of New Zealand white rabbits. Changed in mucosa thickness before and 3 months after operation were compared. The in vivo metabolism and regeneration effect of each group were observed by histological hematoxylin-eosin (HE) and Masson staining. Results SEM showed that the three groups of scaffolds were all cross-linked porous structures. XRD and FTIR showed that the three scaffolds were dominated by a relatively stable Silk Ⅱ structure, which degraded more slowly in vitro. Among them, SF3 had the largest pore size (133.40 μm±22.85 μm) and moderate porosity (90.05%±6.68%). In vivo results showed that the thickening effect of SF1 was similar to that of the control group because of insufficient space-maintenance property. Meanwhile, the properties of SF3 and SF5 were more stable, and the thickening effect was significantly better than those of the control group. However, unlike SF5 that induced obvious inflammation, SF3 showed better degradation, more fibrosis and angiogenesis, and less inflammatory response in vivo. Conclusion Silk-fibroin scaffolds can be applied to effectively thicken soft tissues, among which SF3 (3 wt%) silk fibroin scaffold exhibited the best physicochemical properties, histocompatibility, and mucosal-thickening effect.

Key words: scaffold, silk fibroin, soft tissue thickening, animal experiment

中图分类号:

R 782.2

李德雄, 曹润源, 陈江. 丝素蛋白多孔支架用于口腔软组织增厚的初步研究[J]. 华西口腔医学杂志, 2022, 40(5): 513-521.

Li Dexiong, Cao Runyuan, Chen Jiang. Preliminary study of silk fibroin porous scaffold for oral soft-tissue thickening[J]. West China Journal of Stomatology, 2022, 40(5): 513-521.

图/表 6

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丝素蛋白多孔支架用于口腔软组织增厚的初步研究

Preliminary study of silk fibroin porous scaffold for oral soft-tissue thickening

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