华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (1): 59-68.doi: 10.7518/hxkq.2020.01.011

• 系统评价专栏 • 上一篇    下一篇

干细胞局部应用对缺损面神经再生效果的系统评价

赵丹,李月恒,杨正艳,蔡婷,吴晓艳,夏雨,周智()   

  1. 重庆医科大学附属口腔医院预防口腔科 口腔疾病与生物医学重庆市重点实验室 重庆市高校市级口腔生物医学工程重点实验室,重庆 401120
  • 收稿日期:2019-06-21 修回日期:2019-09-19 出版日期:2020-02-01 发布日期:2020-02-06
  • 通讯作者: 周智 E-mail:500119@hospital.cqmu.edu.cn
  • 作者简介:赵丹,硕士,E-mail: 2017110916@stu.cqmu.edu.cn
  • 基金资助:
    重庆高校创新团队建设计划(CXTDG201602006)

Effect of the local application of stem cells on repairing facial nerve defects: a systematic review

Zhao Dan,Li Yueheng,Yang Zhengyan,Cai Ting,Wu Xiaoyan,Xia Yu,Zhou Zhi()   

  1. Dept. of Preventive Stomatology, Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401120, China
  • Received:2019-06-21 Revised:2019-09-19 Online:2020-02-01 Published:2020-02-06
  • Contact: Zhi Zhou E-mail:500119@hospital.cqmu.edu.cn
  • Supported by:
    Program for Innovation Team Building at Institutions of Higher Education in Chongqing(CXTDG201602006)

摘要:

目的 系统评价干细胞对面神经缺损的修复效果。方法 在Pubmed、Cochrane Library、Web of Science、Embase、Scopus及中国生物医学文献数据库检索关于评价干细胞对动物面神经再生效果的所有原始研究,2名专业人员独立完成文献筛选、数据提取及偏倚风险评估。使用RevMan 5.3软件及随机效应模型进行统计分析,分析结果以均数差(MD)及95%可信区间(CI)的形式呈现。对面神经的功能性评估(胡须运动评分、面瘫评分)及组织学评估(有髓纤维密度、纤维直径、髓鞘厚度、G比值)结果进行Meta分析。结果 从6个数据库共检索出4 614篇文献,15篇被纳入了Meta分析。干细胞组的胡须运动评分、面瘫评分、有髓纤维密度、髓鞘厚度均高于非干细胞组(P<0.05),G比值小于非干细胞组(P=0.001),纤维直径二者无统计学差异(P=0.08)。结论 干细胞具有促进面神经再生的潜能。

关键词: 干细胞, 面神经, 神经组织工程, 系统评价

Abstract:

Objective To systematically evaluate the repairing effect of stem cells on facial nerve defects. Methods Articles regarding the regenerating effect of stem cells on facial nerves in animals were collected from the databases of Pubmed, Cochrane Library, Web of Science, Embase, Scopus, and CBM. Two professionals independently completed the article screening, data extraction, and bias risk assessment. RevMan 5.3 and random-effects models were used for the statistical analysis, and the results were presented in the form of mean differences (MD) with a 95%CI. The results of functional evaluation (vibrissae movement, facial paralysis) and histological evaluation (density of myelinated fibers, diameter of fibers, thickness of myelin sheath, G ratio) of facial nerve were Meta-analyzed. Results A total of 4 614 articles were retrieved from the 6 databases, and 15 of these articles were included in the Meta-analysis. For vibrissae movement and facial paralysis, the stem cell group scored significantly higher than the non-stem cell group (P<0.05). The density of myelinated fibers and thickness of the myelin sheath in the stem cell group were higher than those in the non-stem cell group (P<0.05). The G ratio in the stem cell group was smaller than that in the non-stem cell group (P=0.001). There was no significant difference in fiber diameter (P=0.08). Conclusion Stem cells have potential in promoting facial nerve regeneration.

Key words: stem cell, facial nerve, neural tissue engineering, systematic review

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