华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (6): 613-618.doi: 10.7518/hxkq.2018.06.006

• 基础研究 • 上一篇    下一篇

口腔白斑和口腔鳞状细胞癌中微管相关蛋白1轻链3和雷帕霉素靶蛋白的表达及临床意义

董小琳,柳志文()   

  1. 中南大学湘雅二医院口腔医学中心,长沙 410011
  • 收稿日期:2018-01-20 修回日期:2018-09-29 出版日期:2018-12-01 发布日期:2018-12-12
  • 通讯作者: 柳志文 E-mail:liuzw5048@aliyun.com
  • 作者简介:董小琳,硕士,E-mail: 1404091465@qq.com

Clinical importance of microtubule-associated protein 1 light chain 3 and mammalian target of rapamycin expression in oral leukoplakia and oral squamous cell carcinoma

Xiaolin Dong,Zhiwen Liu()   

  1. Stomatological Center, Xiangya Second Hospital of Central South University, Changsha 410011, China
  • Received:2018-01-20 Revised:2018-09-29 Online:2018-12-01 Published:2018-12-12
  • Contact: Zhiwen Liu E-mail:liuzw5048@aliyun.com

摘要:

目的 探究自噬相关基因微管相关蛋白1轻链3(LC3)和雷帕霉素靶蛋白(mTOR)在正常口腔黏膜、口腔白斑(OLK)及口腔鳞状细胞癌(OSCC)中的表达情况及相互关系,评估其作为OLK恶变早期诊断指标的临床价值。方法 采用免疫组化法检测LC3B和mTOR在20例正常口腔黏膜组织、120例OLK及30例OSCC组织中的表达,回顾性分析120例OLK患者的临床资料,探讨LC3B和mTOR及其与各临床因素之间的关系。结果 正常口腔黏膜、OLK和OSCC组中LC3B阳性率分别为85.0%、65.8%和33.3%(P<0.05),mTOR的阳性率分别为20.0%、48.3%和76.7%(P<0.05)。LC3B与mTOR二者间的表达具有相关性(P<0.05),且其表达都与OLK的临床类型相关(P<0.05)。结论 LC3B和mTOR可作为早期监测口腔OLK恶变的分子生物学指标。

关键词: 微管相关蛋白1轻链3, 雷帕霉素靶蛋白, 白斑

Abstract:

Objective This study aimed to investigate the expression and relationship of microtubule-associated protein 1 light chain 3 (LC3) and mammalian target of rapamycin (mTOR) in normal oral mucosa, oral leukoplakia (OLK), and oral squamous cell carcinoma (OSCC). This work also analyzed the relationship between expression levels and clinical factors. This study evaluated the clinical value of LC3B and mTOR as indices to determine the carcinogenic potential of OLK.Methods Immunohistochemistry was used to detect the expression of LC3B and mTOR in 20 cases of normal oral mucosa, 120 cases of OLK, and 30 cases of OSCC. The clinical data of 120 patients with OLK were analyzed. The relationships between expression levels and clinical factors were investigated.Results In normal oral mucosa, OLK and OSCC, the positive rates of LC3B expression were 85.0%, 65.8% and 33.3% (P<0.05), whereas the positive rates of mTOR expression were 20.0%, 48.3% and 76.7% (P<0.05). The expression levels of LC3B and mTOR were correlated and related to clinical typing of OLK (P<0.05).Conclusion LC3B and mTOR can be used as molecular biomarkers for early detection of OLK.

Key words: microtubule-associated protein 1 light chain 3, mammalian target of rapamycin, leukoplakia

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