浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究

doi:10.7518/hxkq.2018.03.003

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (3): 247-251.doi: 10.7518/hxkq.2018.03.003

浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究

宦俊^1,^2,³(), 窦磊^1,^2,³, 严崎方^1,^2,³, 杨德琴^1,^2,³()

1.重庆医科大学附属口腔医院牙体牙髓科
2.口腔疾病与生物医学重庆市重点实验室
3.重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2017-09-25 修回日期:2018-03-27 出版日期:2018-06-01 发布日期:2018-06-01
作者简介:
宦俊,住院医师,硕士,E-mail：275790331@qq.com
基金资助:
国家自然科学基金面上项目（31571508,31371473）;2016年重庆高校创新团队建设计划;重庆市高校市级口腔生物医学工程重点实验室资助项目[渝教科2014（55）号]

An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells

Jun Huan^1,^2,³(), Lei Dou^1,^2,³, Qifang Yan^1,^2,³, Deqin Yang^1,^2,³()

1. Dept. of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2017-09-25 Revised:2018-03-27 Online:2018-06-01 Published:2018-06-01
Supported by:
The National Natural Science Foundation of China (31571508, 31371473);Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education[2014(55)].

摘要/Abstract

摘要：

目的研究浓缩生长因子（CGF）对人脐静脉血管内皮细胞（HUVECs）的增殖、迁移和分化的作用。方法取健康志愿者静脉血制成CGF,再用CGF制备浓缩生长因子提取液（CGFe）。体外培养细胞分为2%CGFe组、5%CGFe组、10%CGFe组和对照组。CCK-8和细胞周期实验检测各组细胞增殖活性;划痕实验检测内皮细胞迁移;实时荧光定量聚合酶链反应（qRT-PCR）检测各组细胞血管内皮生长因子（VEGF）、趋化因子受体4（CXCR4）、血小板衍生因子（PDGF）mRNA表达量。结果 CCK-8法和细胞周期结果显示CGFe明显促进细胞增殖（P<0.05）,且增殖效应呈CGFe浓度依赖性,各组间均有统计学差异（P<0.05）;划痕实验中12 h时实验组划痕愈合效率明显高于对照组,且愈合效率与CGFe浓度呈正比（P<0.05）;CGFe明显促进VEGF、CXCR4、PDGF的mRNA表达量,促进效应与浓度呈正比（P<0.05）。结论 CGFe能有效促进HUVECs的增殖、迁移和成血管分化。

关键词: 浓缩生长因子, 人脐静脉血管内皮细胞, 增殖, 分化

Abstract:

Objective This study aimed to explore the effects of concentrate growth factor extracts (CGFe) on human umbi-lical vein endothelial cells (HUVECs) in vitro. Methods Concentrate growth factor (CGF) were prepared from the peripheral blood of healthy donors, followed by CGFe. Four groups were designed based on cell culture medium, as follows: 2%CGFe, 5%CGFe, 10%CGFe, and control. The proliferation activity of HUVECs was detected by cell cycle and CCK-8 assays. The migration of HUVECs was detected by scratch assay. The mRNA expression levels of vascular endothelial growth factor (VEGF), chemokine receptor 4 (CXCR4), and platelet derived growth factor (PDGF) were examined by quantitative real time polymerase chain reaction (qRT-PCR). Results Results of CCK-8 and cell cycle assays showed that CGFe promoted the proli-feration capability of HUVECs in a dose-dependent manner, and the data had statistical significance among four groups (P<0.05). The cell migration assay indicated that CGF accelerated wound closure in a dose-dependent manner after 12 h of culture (P<0.05). The results of qRT-PCR showed that CGF upregulated the expression levels of VEGF, CXCR4, and PDGF in HUVECs. Conclusion CGFe can promote the proliferation, migration, and angiogenic differentiation of HUVECs. Thus, CGF might be an appropriate cure for dental pulp revascularization.

Key words: concentrate growth factor, human umbilical vein endothelial cells, proliferation, differentiation

中图分类号:

宦俊, 窦磊, 严崎方, 杨德琴. 浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究[J]. 华西口腔医学杂志, 2018, 36(3): 247-251.

Jun Huan, Lei Dou, Qifang Yan, Deqin Yang. An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells[J]. West China Journal of Stomatology, 2018, 36(3): 247-251.

图/表 5

参考文献 17

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浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究

An in vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells

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