偏侧咀嚼致大鼠咬肌肌纤维代谢特征改变及其腺苷酸活化蛋白激酶调节机制

doi:10.7518/hxkq.2017.03.006

华西口腔医学杂志 ›› 2017, Vol. 35 ›› Issue (3): 258-263.doi: 10.7518/hxkq.2017.03.006

偏侧咀嚼致大鼠咬肌肌纤维代谢特征改变及其腺苷酸活化蛋白激酶调节机制

石安迪¹(), 曾林¹, 刘静²()

1.暨南大学口腔医学院
2.口腔修复学教研室,广州 510632

收稿日期:2016-06-22 修回日期:2017-03-10 出版日期:2017-06-05 发布日期:2017-06-01
作者简介:
石安迪,住院医师,硕士,E-mail：505256764@qq.com
基金资助:
暨南大学优秀人才基金（51025036）

Alteration of metabolic characteristics on the masseter muscle fiber of unilateral chewing rats and its adenosine mono-phosphate activated protein kinase regulatory mechanism

Andi Shi¹(), Lin Zeng¹, Jing Liu²()

1. College of Stomatology, Jinan University, Guangzhou 510632, China
2. Dept. of Prosthodontics, College of Stomatology, Jinan University, Guang-zhou 510632, China

Received:2016-06-22 Revised:2017-03-10 Online:2017-06-05 Published:2017-06-01
Supported by:
Supported by: Talent Fund of Jinan University(51025036).

摘要/Abstract

摘要：

目的探讨偏侧咀嚼对大鼠咬肌肌纤维代谢特征的影响及腺苷酸活化蛋白激酶（AMPK）对肌纤维代谢特征变化的调控作用。方法拔除大鼠右上颌磨牙建立偏侧咀嚼大鼠模型,实验组分为2、4、6、8周组,设同期对照组。应用辅酶Ⅰ-四氮唑还原酶（NADH-TRase）染色法检测咬肌肌纤维有氧代谢类型以及各型肌纤维的分布密度和构成比;用Western blot技术检测p-AMPK（Thr172）/AMPKα1表达水平。结果与同期对照组比较,建模2周实验组拔牙侧深染型肌纤维比例增多,非拔牙侧中染型纤维比例增加,浅染型肌纤维比例减少（P<0.05）;建模4、6、8周组双侧深染型肌纤维比例持续增加,拔牙侧显著高于非拔牙侧;第6、8周组双侧中染型肌纤维比例下降（P<0.05）;拔牙侧浅染肌纤维比例在8周组减少,非拔牙侧无明显改变（P>0.05）。p-AMPK（Thr172）/AMPKα1表达水平在2、4周拔牙侧较对照组和非拔牙侧增高（P<0.05）,在非拔牙侧各周组无显著改变（P>0.05）。结论偏侧咀嚼可促进双侧咬肌肌纤维有氧代谢能力且伴随表型特征的改变,在非工作侧更明显;肌纤维有氧代谢能力增强与AMPK通路的激活有关。

关键词: 辅酶Ⅰ, -四氮唑还原酶, 腺苷酸活化蛋白激酶, 氧化代谢, 偏侧咀嚼

Abstract:

Objective This study aims to determine the influence of unilateral chewing on metabolic characteristics of masseter muscle fibers in rats and the regulatory effect of an adenosine monophosphate activated protein kinase (AMPK) signal pathway on metabolism. Methods Rats were submitted to exodontia of all the right maxillary molars and divided into 2, 4, 6, and 8 weeks groups, and corresponding control groups were set as well. Sections were stained by nicotine adenine dinucleotide tetrazolim reductase(NADH-TRase) to demonstrate the types, proportion, and density of masseter muscle fibers. AMPKα1 and p-AMPK(Thr172) levels in bilateral masseter muscles were detected by Western blot. Results In the 2-week group, the percentage of dark fibers augmented in the ipsilateral side, whereas the percentage of intermediary fibers in the contralateral side was increased accompanied by a decrease of light fibers, compared with the control group (P<0.05). The percentage of dark fibers was increased in the bilateral sides, whereas the percentage of dark fiber in the ipsilateral sides surpassed that of the contralateral sides in the 4, 6, and 8-week groups. The percentage of intermediary fibers was decreased in the bilateral sides in the 6 and 8-week groups (P<0.05). The percentage of light fibers was reduced in the ipsilateral sides in the 8-week group, whereas no alteration was observed in contralateral sides (P>0.05). In the ipsilateral sides, p-AMPK (Thr172)/AMPKα1 levels were increased in the 2 and 4-week groups (P<0.05), whereas no change was observed in the con-tralateral sides in either group (P>0.05). Conclusion Uni-lateral chewing increases the oxidative metabolic ability in bilateral masseter muscle fibers especially in the non-working side accompanied with change of muscle fiber types. The improvement of aerobic metabolism ability is related to the AMPK signal pathway.

Key words: nicotine adenine dinucleotide tetrazolium reductase, adenosine monophosphate activated protein kinase, oxidative metabolism, unilateral chewing

中图分类号:

Q 257

石安迪, 曾林, 刘静. 偏侧咀嚼致大鼠咬肌肌纤维代谢特征改变及其腺苷酸活化蛋白激酶调节机制[J]. 华西口腔医学杂志, 2017, 35(3): 258-263.

Andi Shi, Lin Zeng, Jing Liu. Alteration of metabolic characteristics on the masseter muscle fiber of unilateral chewing rats and its adenosine mono-phosphate activated protein kinase regulatory mechanism[J]. West China Journal of Stomatology, 2017, 35(3): 258-263.

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时间	组别	深染	中染	浅染
2周	实验组拔牙侧	26.07±1.02^acde	43.02±2.71^de	30.59±2.59^e
	实验组非拔牙侧	22.31±1.93	54.30±1.47^acd	22.40±1.57^acd
	对照组右侧	20.59±2.10	45.87±2.87	33.53±1.16
	对照组左侧	21.65±1.15	45.03±2.99	33.43±1.83
4周	实验组拔牙侧	31.33±2.26^abde	41.79±2.84^ade	27.52±5.14
	实验组非拔牙侧	26.08±1.69^a	48.31±0.97^bd	25.63±2.85^abd
	对照组右侧	20.98±1.77	45.43±1.60	33.58±1.31
	对照组左侧	19.89±2.17	46.69±1.56	33.40±2.40
6周	实验组拔牙侧	39.90±1.94^abce	30.86±1.41^ae	29.84±5.26
	实验组非拔牙侧	26.59±4.36	40.32±1.58^abc	32.97±2.17^bc
	对照组右侧	21.57±1.87	44.82±3.02	33.63±1.78
	对照组左侧	20.37±2.02	46.25±2.98	33.37±1.36
8周	实验组拔牙侧	35.20±2.82^abcde	39.97±4.98^ad	24.33±1.57^abe
	实验组非拔牙侧	26.08±1.69^a	40.92±2.87^abc	33.27±1.10^bc
	对照组右侧	20.45±2.64	46.81±1.23	32.73±1.70
	对照组左侧	19.68±1.17	47.91±2.15	32.40±2.33

组别	2周	4周	6周	8周
对照组	1	0.99±0.07	1.05±0.11	1.14±0.04
实验组非拔牙侧	0.77±0.33	1.19±0.26	1.22±0.36	1.28±0.55
实验组拔牙侧	1.50±0.17^ade	1.31±0.30^a	1.12±0.29^b	0.87±0.11^bcd

偏侧咀嚼致大鼠咬肌肌纤维代谢特征改变及其腺苷酸活化蛋白激酶调节机制

Alteration of metabolic characteristics on the masseter muscle fiber of unilateral chewing rats and its adenosine mono-phosphate activated protein kinase regulatory mechanism

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