华西口腔医学杂志

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马尾松松针提取物对根面牙本质脱矿影响的体外研究

唐成芳1 阮建平2 朱勇1 李子夏1 左艳萍1 徐红艳3   

  1. 1.西安医学院口腔医学系，西安 710021；2.西安交通大学口腔医院预防科，西安 710004；3.陕西省人民医院口腔科，西安 710076
  • 收稿日期:2016-03-15 修回日期:2016-06-22 出版日期:2016-10-01 发布日期:2016-10-01
  • 通讯作者: 唐成芳，副教授，博士，E-mail：tangchengfang@sohu.com
  • 作者简介:唐成芳，副教授，博士，E-mail：tangchengfang@sohu.com
  • 基金资助:

    陕西省科技厅社会发展项目（2015SF242）；陕西省教育厅科研课题（15JK1625）；西安医学院博士启动基金（2015DOC20）；西安医学院重点学科资助

Effect of Pinus massoniana needle extract on root dentin demineralization in vitro

Tang Chengfang1, Ruan Jianping2, Zhu Yong1, Li Zixia1, Zuo Yanping1, Xu Hongyan3.   

  1. 1. Dept. of Stomatology, Xi’an Medical University, Xi’an 710021, China; 2. Dept. of Preventive, School of Stomatology, Xi’an Jiao Tong University, Xi’an 710004, China; 3. Dept. of Stomatology, Shanxi Provincal People’s Hospital, Xi’an 710076, China
  • Received:2016-03-15 Revised:2016-06-22 Online:2016-10-01 Published:2016-10-01
  • Contact: Tang Chengfang, E-mail: tangchengfang@sohu.com.
  • Supported by:

    Tackling Project of Scientific and Technological Social Development from Science and Technology Department of Shanxi Province(2015SF242); Scientific Research Project from Education Department of Shanxi Province (15JK1625); Doctor Start Fund Project from Xi’an Medical University (2015DOC20); Key Discipline from Xi’an Medical University.

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摘要：

目的 评价马尾松松针提取物（PMNE）抑制根面牙本质脱矿的效果。方法 将根面牙本质块根据pH循环中所用实验溶液随机分为去离子蒸馏水（DDW）组，氟化钠（NaF）组，4%、8%和12%PMNE组，试件进行为期8 d的pH循环。显微CT选区测定各组未脱矿牙本质和脱矿牙本质的牙本质矿物密度（DMD）及两者DMD差值（ΔDMD），扫描电镜下观察pH循环后牙本质表面形貌。结果 PMNE各组和NaF组ΔDMD均显著低于DDW组（P<0.05），且8%PMNE组和12%PMNE组ΔDMD组间差异无统计学意义（P>0.05），均显著低于4%PMNE组，高于NaF组（P<0.05）。扫描电镜见PMNE组牙本质小管不全暴露，小管开口程度明显小于DDW组，大于NaF组。结论 PMNE可抑制根面牙本质脱矿，减缓酸对DMD的降低，具有一定的抗龋活性。8%PMNE即可达到较好抑制脱矿的效果。

关键词: 马尾松松针提取物, 根面龋, 牙本质, 脱矿, 矿物密度

Abstract:

Objective  This study aims to evaluate the effects of Pinus massoniana needle extract (PMNE) on inhibiting demineralization of root dentin. Methods  Root dentin blocks were randomly divided into distilled deionized water (DDW) group, fluoride sodium (NaF) group, and 4%, 8% and 12% PMNE groups according to the experimental solution used in the process of pH cycling in each group. All specimens in each group experienced pH cycling for 8 d. The dentin mineral density (DMD) of the normal dentin and demineralized dentin and their D-value (ΔDMD) were determined using micro computed tomography. The morphology of dentin surface after pH cycling was also observed using a scanning electron microscope. Results The ΔDMD values in all PMNE groups and the NaF group were considerably lower than the ΔDMD in the DDW group (P<0.05). The ΔDMD values of the 8% and 12% PMNE groups had no difference (P>0.05), both of which were lower than the ΔDMD in the 4% PMNE group and higher than that in the NaF group (P<0.05). The dentin tubules were partly opened in the PMNE groups. The opening degrees of the dentin tubule in PMNE groups were significantly less and smaller than the opening degree in the DDW group and were larger than that in the NaF group. Conclusion  PMNE can inhibit the demineralization of root dentin and can slow down the reduction in DMD. PMNE has the potential to prevent caries, and 8% PMNE can effectively inhibit dentin demineralization.

Key words: Pinus massoniana needle extract, root caries, dentin, demineralization, mineral density

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