华西口腔医学杂志

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牙髓卟啉单胞菌在原发性感染和再感染根管内的定植

李红 纪海 何艳艳 杨圣辉 侯本祥   

  1. 首都医科大学附属北京口腔医院牙体牙髓科,北京 100050
  • 出版日期:2015-02-01 发布日期:2015-02-01
  • 通讯作者: 侯本祥,教授,博士,E-mail:houbenxiang@gmail.com
  • 作者简介:李红,副主任医师,博士,E-mail:lihongdentist@163.com
  • 基金资助:

    国家自然科学基金资助项目(81170952);北京市自然科学基金资助项目(7132111)

Colonization of Porphyromonas endodontalis in primary and secondary endodontic infections

Li Hong, Ji Hai, He Yanyan, Yang Shenghui, Hou Benxiang   

  1. Dept. of Endodontics, School of Stomatology, Capital Medical University, Beijing 100050, China
  • Online:2015-02-01 Published:2015-02-01

摘要:

目的  采用16s rDNA PCR和实时荧光定量聚合酶链反应(RTFQ-PCR)分析和比较牙髓卟啉单胞菌(P. endodontalis)endodontalis)在原发性感染和再感染根管中的定植情况。方法  将120例慢性根尖周炎患者的120颗单根管患牙按原发性感染和再感染分为2组,每组60颗。采用16s rDNA PCR分析P. endodontalis在两种感染根管内的检出率,对于P. endodontalis endodontalis检出阳性者用RTFQ-PCR比较P. endodontalis在两种感染根管内的DNA相对表达量。结果  P. endodontalis在原发性感染根管内的检出率明显高于再感染根管的检出率(P=0.001)。RTFQ-PCR检测结果表明P. endodontalis在原发性感染根管内的DNA表达量和再感染根管内DNA表达量差异无统计学意义(P=0.303)。结论  P. endodontalis在原发性感染和再感染根管内均有定植,但与原发性感染根管关系更为密切。

关键词: 牙髓卟啉单胞菌, 原发性感染根管, 再感染根管, 慢性根尖周炎, 实时荧光定量聚合酶链反应

Abstract:

Objective  This study aims to assess and compare the prevalence of Porphyromonas endodontalis (P. endodontalis) in root canals associated with primary and secondary endodontic infections by using 16s rDNA PCR and real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Methods  A total of 120 adult patients with one radiographically documented periapical lesion were included. Sixty teeth presented with primary endodontic infections and 60 with secondary endodontic infections requiring retreatment. P. endodontalis was identified by using 16s rDNA PCR techniques. The positive DNA expression of P. endodontalis in two types of infected root canals were quantitatively compared by using SYBR GREEN Ⅰ RTFQ-PCR. Results  The prevalence of P. endodontalis in the root canals with primary endodontic infections was significantly higher than that in root canals with secondary endodontic infections (P=0.001). However, RTFQPCR results showed no significant difference in DNA expression quantities between the primary and secondary endodontic infections root canals (P=0.303). Conclusion  P. endodontalis is more highly associated with root canals having primary endodontic infections, although P. endodontalis colonize in both root canals with primary and secondary chronic apical periodontitis.

Key words: Porphyromonas endodontalis, primary endodontic infection, secondary endodontic infection, chronic apical periodontitis, real-time fluorescence quantitative polymerase chain reaction