华西口腔医学杂志

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层层静电自组装构建载药种植体的研究

徐倩1 冯青2 欧俊3 孙红1   

  1. 1.芜湖市第一人民医院口腔科,芜湖 241000;2.广西医科大学口腔医院种植科,南宁 530021;3.桂林理工大学材料科学与工程学院,有色金属及材料加工新技术教育部重点实验室,桂林 541004
  • 出版日期:2014-12-01 发布日期:2014-12-01
  • 通讯作者: 冯青,副教授,硕士,E-mail:fengqing302@sina.com
  • 作者简介:徐倩,住院医师,硕士,E-mail:xuqian2012@gmail.com
  • 基金资助:

    广西医疗卫生重点科研课题基金资助项目(重2010083)

Construction of drug-loaded titanium implants via layer-by-layer electrostatic self-assembly

Xu Qian1, Feng Qing2, Ou Jun3, Sun Hong1.   

  1. 1. Dept. of Stomatology, Wuhu First People’s Hospital, Wuhu 241000, China; 2. Dept. of Implantology, School of Stomatology, Guangxi Medical University, Nanning 530021, China; 3. Materials Science and Engineering College of Guilin University of Technology, Key Lab of New Processing Technology for Nonferrous Material Ministry of Education, Guilin 541004, China
  • Online:2014-12-01 Published:2014-12-01

摘要:

目的 构建一种长效、靶向的携带促骨形成药物HU-308的药物缓释种植体,观察其体外缓释特性。方法 采用层层静电自组装技术制备不同层数的肝素/壳聚糖涂层,以物理吸附的方式装载促骨形成药物HU-308,构建载药种植并进行体外释放实验。通过紫外可见光分光光度计测定药物浓度,分析不同涂层数载药种植体的加载效率及释放规律。用扫描电镜、原子力显微镜观察涂层表面形貌和结构的变化。结果 成功地制备了载HU-308涂层种植体。体外释放实验表明,随着涂层层数的增加,载药量逐渐增加,但T20组略有下降。随着层数的增加,HU-308的释放速度随之减缓,缓释能力增强。扫描电镜、原子力显微镜结果表明种植体表面肝素/壳聚糖涂层逐渐形成。结论 层层静电自组装技术成功构建载有HU-308的涂层种植体,可长期有效释放达30 d以上,这有望为临床上提高骨质疏松症患者种植体骨整合率提供一种新的可能。

关键词: 钛种植体, 药物缓释, HU-308, 层层静电自组装, 肝素, 壳聚糖

Abstract:

Objective  This study aims to construct a long-term, osteogenesis-targeting HU-308 drug delivery implant by the layer-by-layer electrostatic self-assembly (LBL) technique, and observe the features of its delayed release in vitro. Methods  A heparin (Hep) and chitosan (Chi) multilayer was coated on pure titanium using the LBL technique, and the titanium implants were dipped into the solution to load HU-308. The amount of loaded drug and release rates were measured using a UV-Vis spectrophotometer. The relationships between the loading efficiency, release time, and multilayer films were evaluated. The morphology of all the multilayers was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results  The results showed that the Hep/Chi self-assembly multilayer was gradually fabricated on the titanium surface. HU-308 was successfully loaded on the titanium implants. The amount of loaded drug increased with the amount of multilayer films, except in the T20 group. In vitro drug release study showed that drug release was more difficult in the system with thicker films, and large amounts of multilayer films decreased the release speed. Both SEM and AFM measurements showed typical LBL deposition of Hep and Chi. Conclusion  The HU-308 drug delivery implant is successfully fabricated via LBL technology. It could provide sustained release of HU-308 over 30 d. This type of implant may provide a new possibility of promoting implant–bone osseointegration for osteoporotic patients.

Key words: titanium implant, drug delivery, HU-308, layer-by-layer electrostatic self-assembly, heparin, chitosan